清喉利咽颗粒的UPLC指纹图谱建立及主成分分析

发布时间：2018-10-10 07:57

【摘要】：目的:建立清喉利咽颗粒的超高效液相色谱(UPLC)指纹图谱,并结合主成分分析法(PCA)为其质量控制提供参考。方法:采用UPLC法。色谱柱为ACQUITY UPLC BEH C18,流动相为乙腈(A)-0.1%磷酸水溶液(B)(梯度洗脱),流速为0.4 m L/min,检测波长为280 nm,柱温为30℃,进样量为2μL。以鞣花酸为参照物,分析12批清喉利咽颗粒样品,采用"中药指纹图谱相似度评价系统"软件进行相似度分析,并根据对照品对其中的共有峰进行指认,同时对共有峰进行主成分分析。结果:12批清喉利咽颗粒指纹图谱标定了18个共有峰,指认了6个主要色谱峰(没食子酸、鞣花酸、柚皮苷、橙皮苷、新橙皮苷、黄芩苷);12批样品与对照指纹图谱相似度均≥0.984。经主成分分析,18个共有峰可综合为3个主成分,累计方差贡献率为78.277%;黄芩苷和16号峰为清喉利咽颗粒指纹图谱中影响比较大的指标。结论:该方法可为清喉利咽颗粒的质量评价提供参考。
[Abstract]:Objective: to establish the (UPLC) fingerprint of Qinghaliyan granules by ultra-high performance liquid chromatography (HPLC), and to provide reference for its quality control with principal component analysis (PCA). Methods: UPLC method was used. The chromatographic column was ACQUITY UPLC BEH C18, the mobile phase was (B) (gradient elution of acetonitrile (A) -0.1% phosphoric acid solution), the flow rate was 0.4 mL / min, the detection wavelength was 30 鈩，

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