基于生物素与亲和素、二硫键的宏观自组装研究
发布时间：2018-10-12 12:16
【摘要】：超分子相互作用和形成共价键是进行自组装的主要手段和方法。利用分子间弱的相互作用力是超分子组装的根本,可以实现大量构筑基元在温和条件下进行简单快速,厚度、形貌、成分可控的平行组装,为超分子组装迈向工业化奠定了基础。但是超分子组装仍存在组装力小、主客体分子生物毒性大、不同超分子力间相互干扰等缺点,所以我们分别选择生物相容性的Biotin/Avid in系统、可逆的S-S共价键氧化还原反应作为宏观自组装的作用力。生物素/亲和素是已知作用力最强的非共价键,并且特异性强、生物相容性好,可以运用到生物相容性组织工程直接的构筑上;S-S可以在不同的条件下进行氧化还原反应,实现宏观物体可逆的组装/解组装,其特点就是利用共价键作用力强实现更稳定组装体,并且我们使用带SH的半胱氨酸作为功能分子,本身具有很好的生物相容性,可以为生物相容性组织工程支架的构筑提供新的方法。我们首先制作PDMS小块作为自组装的宏观基元,利用酰胺化接枝反应在EDC/NHS的活化催化下于水相中分别合成了PAH-Biotin、PAH-SH等带有特定官能团的聚电解质,通过Layer-by-Layer方法修饰到PDMS小块表面,使之带有不同的组装官能团,在特定的条件下实现宏观基元的自组装。为实现生物相容性的、可逆的、稳定的、高选择性的宏观自组装提供了一些新的研究方向。
[Abstract]:Supramolecular interaction and formation of covalent bonds are the main means and methods for self-assembly. The use of weak intermolecular interaction is fundamental to the supramolecular assembly, which can realize a large number of elements to be constructed in a simple, rapid, thickness, morphology, and controllable composition parallel assembly under mild conditions. It lays the foundation for supramolecular assembly to industrialization. However, supramolecular assembly still has some disadvantages, such as low assembly power, high toxicity of host and guest molecules, interference between different supramolecular forces, so we choose biocompatible Biotin/Avid in system. The reversible S S covalent redox reaction acts as a macro self assembly force. Biotin / avidin is a non-covalent bond with the strongest known force, and it has strong specificity and good biocompatibility, which can be used in direct construction of biocompatibility tissue engineering. S-S can be redox under different conditions. To achieve reversible assembly / unassembly of macroscopic objects, it is characterized by the use of covalent bond forces to achieve a more stable assembly, and we use cysteine with SH as functional molecules with good biocompatibility. It can provide a new method for the construction of biocompatibility tissue engineering scaffolds. In this paper, we first prepared PDMS fragments as macroelements for self-assembly. Polyelectrolytes such as PAH-Biotin,PAH-SH with specific functional groups were synthesized in aqueous phase by amidation graft reaction under the catalysis of EDC/NHS activation, and then modified to the surface of PDMS fragments by Layer-by-Layer method. It has different assembly functional groups and realizes the self-assembly of macro-elements under certain conditions. It provides some new research directions for achieving biocompatible, reversible, stable and highly selective macro-self-assembly.
【学位授予单位】：北京化工大学
【学位级别】：硕士
【学位授予年份】：2016
【分类号】：O641.3
本文编号：2266082
[Abstract]:Supramolecular interaction and formation of covalent bonds are the main means and methods for self-assembly. The use of weak intermolecular interaction is fundamental to the supramolecular assembly, which can realize a large number of elements to be constructed in a simple, rapid, thickness, morphology, and controllable composition parallel assembly under mild conditions. It lays the foundation for supramolecular assembly to industrialization. However, supramolecular assembly still has some disadvantages, such as low assembly power, high toxicity of host and guest molecules, interference between different supramolecular forces, so we choose biocompatible Biotin/Avid in system. The reversible S S covalent redox reaction acts as a macro self assembly force. Biotin / avidin is a non-covalent bond with the strongest known force, and it has strong specificity and good biocompatibility, which can be used in direct construction of biocompatibility tissue engineering. S-S can be redox under different conditions. To achieve reversible assembly / unassembly of macroscopic objects, it is characterized by the use of covalent bond forces to achieve a more stable assembly, and we use cysteine with SH as functional molecules with good biocompatibility. It can provide a new method for the construction of biocompatibility tissue engineering scaffolds. In this paper, we first prepared PDMS fragments as macroelements for self-assembly. Polyelectrolytes such as PAH-Biotin,PAH-SH with specific functional groups were synthesized in aqueous phase by amidation graft reaction under the catalysis of EDC/NHS activation, and then modified to the surface of PDMS fragments by Layer-by-Layer method. It has different assembly functional groups and realizes the self-assembly of macro-elements under certain conditions. It provides some new research directions for achieving biocompatible, reversible, stable and highly selective macro-self-assembly.
【学位授予单位】：北京化工大学
【学位级别】：硕士
【学位授予年份】：2016
【分类号】：O641.3
【参考文献】
相关期刊论文 前1条
1 田悦;杜军保;;二硫键和巯基在蛋白质结构功能中的作用及分析方法[J];实用儿科临床杂志;2007年19期
，本文编号：2266082
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