离子液体基聚脂质体的制备、表征及其生物电化学应用

发布时间：2018-10-14 20:14

【摘要】：氧化还原蛋白质在电极上的电化学研究是生物电化学界和生命科学界非常关注的研究问题。它的研究对于研究蛋白质的电子传递机制以及开发新型的无媒介体电化学生物传感器具有重要的意义。本论文利用离子液体基聚脂质体设计和修饰电极表面,探讨了血红素蛋白质的直接电化学性质及其催化特性。这些研究对于构造性能优良的第三代的生物传感器提供了新的思路和机遇。本论文主要做了以下几方面的研究工作：1.本文首先合成了离子液体基脂质体单体以及脂质体。在此基础上,通过自由基聚合反应制备了离子液体基聚脂质体,之后,又对离子液体基聚脂质体进行改进,与氯金酸进行离子交换,制备出金纳米粒子修饰的离子液体基聚脂质体,并利用傅立叶红外光谱、透射电镜和动态光散射技术等对其结构、形貌和表面性质进行了表征。实验结果表明,相对于离子液体基脂质体,聚脂质体在干燥状态下具有较好的结构稳定性,对其改进后,聚脂质体对金纳米粒子也有较好的固定作用,因此可有效保持其固有形貌和结构。2.首次将离子液体基聚脂质体、金纳米粒子修饰的离子液体基聚脂质体与辣根过氧化酶(HRP)结合构筑了HRP/Polysome/PVA/GC、 HRP/Polysome-Au/PVA/GC修饰电极。由于离子液体基聚脂质体兼具离子液体的较好的电子传输性以及脂质体的优良的生物相容性,修饰电极上的HRP不但能够实现与玻碳电极之间的直接电子转移,而且对NaNO2和H2O2表现出很好的电化学催化性质。IRP/Polysome-Au/PVA/GC修饰电极比HRP/Polysome/PVA/GC修饰电极表现出较好的电化学性能,同时还实现了对NaNO2和H202的快速检测。该类生物相容性的纳米复合材料对直接电化学和生物传感器的构筑提供了潜在的应用价值。
[Abstract]:Electrochemical study of redox proteins on electrodes is a subject of great concern in bioelectrochemistry and life sciences. It is of great significance to study the electron transport mechanism of protein and to develop a novel vectorless electrochemical biosensor. In this paper, the direct electrochemical properties and catalytic properties of heme proteins were investigated by designing and modifying the electrode surface with ionic liquid based polyliposomes. These studies provide new ideas and opportunities for constructing the third generation biosensors with excellent performance. This paper mainly does the following research work: 1. In this paper, ionic liquid based liposome monomers and liposomes were synthesized. On this basis, the ionic liquid-based polyliposomes were prepared by free radical polymerization, and then the ionic liquid-based polyliposomes were improved to exchange with chloruronic acid. Gold nanoparticles modified ionic liquid-based polyliposomes were prepared and characterized by Fourier transform infrared spectroscopy (FTIR), transmission electron microscopy (TEM) and dynamic light scattering (DLS) techniques. The experimental results show that polyliposomes have better structural stability than ionic liquid-based liposomes in dry state, and the modified polyliposomes also have a better immobilization effect on gold nanoparticles. Therefore, it can effectively maintain its inherent morphology and structure. 2. The HRP/Polysome/PVA/GC, HRP/Polysome-Au/PVA/GC modified electrode was constructed by combining ionic liquid-based polyliposomes and gold nanoparticles modified ionic liquid polyliposomes with horseradish peroxidase (HRP) for the first time. Due to the good electron transport property of ionic liquids and the excellent biocompatibility of liposomes, the HRP on the modified electrode can not only realize the direct electron transfer with glassy carbon electrode. IRP/Polysome-Au/PVA/GC modified electrode showed better electrochemical performance than HRP/Polysome/PVA/GC modified electrode, and the rapid detection of NaNO2 and H202 was achieved. These biocompatible nanocomposites provide potential applications for direct electrochemistry and biosensors.
【学位授予单位】：辽宁大学
【学位级别】：硕士
【学位授予年份】：2016
【分类号】：O657.1

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