金银花与山银花的HPLC指纹图谱鉴别研究
发布时间：2018-10-15 09:36
【摘要】：目的建立金银花的HPLC对照指纹图谱,并在相同色谱条件下测定山银花的HPLC指纹图谱,通过计算相似度来区分山银花与金银花。方法色谱柱Phenomenex Luna 5μm C18(2)100 A(250 mm×4.6 mm,5μm);柱温40℃,以乙腈-0.5%磷酸溶液为流动相梯度洗脱,体积流量1 m L/min,检测波长为350 nm,相似度计算采取屏蔽大峰积分的方法。结果建立了金银花的HPLC对照指纹图谱,12批金银花相似度均在0.95以上,山银花相似度均小于0.80。结论 350nm下金银花的HPLC指纹图谱能有效区分金银花与山银花药材,屏蔽大峰积分的相似度评价方法比较准确地反映了金银花与山银花间化学成分的差异。
[Abstract]:Objective to establish the HPLC fingerprint of honeysuckle and to determine the HPLC fingerprint of Lonicera japonica under the same chromatographic conditions, and to distinguish the honeysuckle from the honeysuckle by calculating the similarity. Methods the column temperature of Phenomenex Luna 5 渭 m C18 (2) 100A (250 mm 脳 4.6 mm,5 渭 m);) was 40 鈩，
本文编号：2272121
[Abstract]:Objective to establish the HPLC fingerprint of honeysuckle and to determine the HPLC fingerprint of Lonicera japonica under the same chromatographic conditions, and to distinguish the honeysuckle from the honeysuckle by calculating the similarity. Methods the column temperature of Phenomenex Luna 5 渭 m C18 (2) 100A (250 mm 脳 4.6 mm,5 渭 m);) was 40 鈩，
本文编号：2272121
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