激光剥蚀电感耦合等离子体质谱法中生物样品的元素分馏效应研究
发布时间：2018-10-15 11:55
【摘要】：采用213 nm-纳秒激光剥蚀系统对生物基体样品的剥蚀颗粒进行研究,优化了激光剥蚀条件。在剥蚀能量为25%,束斑直径为200μm,剥蚀速率为20μm/s,频率为20 Hz,载气为700 m L He+700 m L Ar时,信号强度及稳定性最佳。以~(31)P为内标元素,最佳剥蚀条件下,考察了56个元素的相对分馏因子。结果表明,生物基体的剥蚀颗粒相较于NIST 610玻璃标样更大,达到3μm;生物基体中元素分馏效应相较于玻璃基体小,大多数元素的相对分馏因子达到1.0±0.1。探讨了生物基体中元素分馏机理,分析了生物基体相较于玻璃基体剥蚀颗粒大,而相对分馏因子未明显增大的原因。一方面可能是粒径3μm的颗粒进入电感耦合等离子体后能原子化;另一方面,大的剥蚀颗粒的富集效应相对较小。进一步对分馏效应的影响因素进行研究,发现分馏效应与激光剥蚀能量、激光频率和扫描速率相关,并且与元素的氧化物沸点负相关,与氧化物键能和电离能正相关。
[Abstract]:The denudation particles of biological matrix samples were studied by 213 nm- nanosecond laser denudation system, and the laser denudation conditions were optimized. When the denudation energy is 25, the beam spot diameter is 200 渭 m, the denudation rate is 20 渭 m / s, the frequency is 20 Hz, the carrier gas is 700 m L He 700 m L Ar, the signal intensity and stability are the best. The relative fractionation factors of 56 elements were investigated under the optimum denudation conditions with ~ (31) P as the internal standard element. The results show that the denudation particles of the biological matrix are larger than the NIST 610 glass standard sample, and the fractionation effect of the elements in the biological matrix is smaller than that of the glass matrix, and the relative fractionation factor of most elements is 1.0 卤0.1. The mechanism of element fractionation in biological matrix was discussed. The reason that the denudation particle of biological matrix was larger than that of glass substrate but the relative fractionation factor was not obviously increased was analyzed. On the one hand, it is possible that the particle size of 3 渭 m is atomized after entering the inductively coupled plasma; on the other hand, the enrichment effect of the large denudated particles is relatively small. It is found that the fractionation effect is related to the laser denudation energy, laser frequency and scanning rate, and negatively correlated with the oxide boiling point of the elements, and positively correlated with the oxide bond energy and ionization energy.
【作者单位】： 中国科学院上海硅酸盐研究所;中国科学院大学;
【基金】：国家自然科学基金项目(No.21175145) 中国科学院仪器设备功能开发技术创新项目(No.Y57YQ5150G)资助~~
【分类号】：O657.63;Q-33
，
本文编号：2272474
[Abstract]:The denudation particles of biological matrix samples were studied by 213 nm- nanosecond laser denudation system, and the laser denudation conditions were optimized. When the denudation energy is 25, the beam spot diameter is 200 渭 m, the denudation rate is 20 渭 m / s, the frequency is 20 Hz, the carrier gas is 700 m L He 700 m L Ar, the signal intensity and stability are the best. The relative fractionation factors of 56 elements were investigated under the optimum denudation conditions with ~ (31) P as the internal standard element. The results show that the denudation particles of the biological matrix are larger than the NIST 610 glass standard sample, and the fractionation effect of the elements in the biological matrix is smaller than that of the glass matrix, and the relative fractionation factor of most elements is 1.0 卤0.1. The mechanism of element fractionation in biological matrix was discussed. The reason that the denudation particle of biological matrix was larger than that of glass substrate but the relative fractionation factor was not obviously increased was analyzed. On the one hand, it is possible that the particle size of 3 渭 m is atomized after entering the inductively coupled plasma; on the other hand, the enrichment effect of the large denudated particles is relatively small. It is found that the fractionation effect is related to the laser denudation energy, laser frequency and scanning rate, and negatively correlated with the oxide boiling point of the elements, and positively correlated with the oxide bond energy and ionization energy.
【作者单位】： 中国科学院上海硅酸盐研究所;中国科学院大学;
【基金】：国家自然科学基金项目(No.21175145) 中国科学院仪器设备功能开发技术创新项目(No.Y57YQ5150G)资助~~
【分类号】：O657.63;Q-33
，
本文编号：2272474
本文链接：https://www.wllwen.com/kejilunwen/huaxue/2272474.html
教材专著
