高效液相色谱-串联质谱法检测红葡萄酒中功效成分

发布时间：2018-10-15 15:11

【摘要】：建立了高效液相色谱-串联质谱法快速测定葡萄酒中白藜芦醇、黄酮类、多酚类功效成分的分析方法。葡萄酒样品直接稀释后进样,用C18柱进行分离,以乙腈-0.1%(体积分数)甲酸水溶液为流动相进行梯度洗脱,通过多反应监测(MRM)模式进行检测。13种功效成分在各自线性范围内呈良好的线性关系,相关系数均大于0.99。除表没食子儿茶素、没食子儿茶素、儿茶素没食子酸酯、花旗松素的检出限为1.0、1.0、3.0、3.0μg/L外,其他9种化合物的检出限均小于1.0μg/L。回收率为80.9%~112.3%,相对标准偏差小于10%。该方法快速、准确、灵敏度高,适用于葡萄酒中功效成分的快速分析。对实际样品的检测表明,所测葡萄酒样品中均含有儿茶素、表儿茶素、表没食子儿茶素、没食子儿茶素、表儿茶素没食子酸酯/儿茶素没食子酸酯、白藜芦醇、大豆黄素等功效成分,不同品种葡萄酒中这些功效成分含量差异显著。
[Abstract]:A rapid method for the determination of resveratrol, flavonoids and polyphenols in wine by high performance liquid chromatography-tandem mass spectrometry (HPLC / MS) was established. The wine samples were directly diluted and separated by C18 column. The mobile phase of acetonitrile-0.1% (volume fraction) formic acid solution was used for gradient elution. The results showed that there was a good linear relationship among the 13 functional components in their linear range, and the correlation coefficients were all greater than 0.99. Except for epigallocatechin, catechin gallate and flagellin, the detection limit was 1.0 渭 g / L, 1.0 渭 g / L and 3.0 渭 g / L respectively, and the detection limits of the other nine compounds were less than 1.0 渭 g / L. The recovery was 80.9 and the relative standard deviation was less than 10. 3%. The method is rapid, accurate and sensitive. It is suitable for rapid analysis of functional components in wine. The results showed that the wine samples contained catechin, epigallocatechin, epigallocatechin / catechin gallate, resveratrol. The content of daidzein and other functional components in different varieties of wine was significantly different.
【作者单位】：中国检验检疫科学研究院食品安全研究所;上海出入境检验检疫局动植物与食品检验检疫技术中心;伊犁出入境检验检疫局综合技术服务中心;
【基金】：国家质检总局科技项目(2015IK311)~~
【分类号】：TS261.7;O657.63

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