花生油和玉米油中多组分真菌毒素高效液相色谱-串联质谱检测方法的建立

发布时间：2018-10-16 12:16

【摘要】：建立花生油和玉米油中黄曲霉毒素、玉米赤霉烯酮、单端孢霉烯族化合物等13种真菌毒素的高效液相色谱-串联质谱检测方法。样品经乙腈-水提取、Multi Sep 226多功能净化柱净化后,分别用电喷雾正离子模式和负离子模式检测。经优化,正离子模式流动相为含2 mmol/L甲酸铵的0.1%甲酸-甲醇溶液,负离子模式流动相为0.1%氨水-乙腈溶液。该方法对花生油和玉米油中13种真菌毒素的检出限为0.05~3.00μg/kg,定量限为0.10~10.00μg/kg,平均加标回收率为66.6%~114.9%。所建方法操作简单、灵敏度高、重复性好,可用于花生油和玉米油中多组分真菌毒素的协同测定。
[Abstract]:A high performance liquid chromatography-tandem mass spectrometry (HPLC / MS) method was developed for the determination of 13 mycotoxins such as aflatoxin, zearalenone and monosporin in peanut oil and corn oil. The samples were purified by acetonitrile-water extraction, Multi Sep 226 multifunctional purification column and detected by electrospray positive ion mode and negative ion mode respectively. The optimized mobile phase was 0.1% formate-methanol solution containing 2 mmol/L ammonium formate, while the anion mode mobile phase was 0.1% ammonia water acetonitrile solution. The detection limit of 13 mycotoxins in peanut oil and corn oil was 0.05 ~ 3.00 渭 g / kg, the quantitative limit was 0.10 ~ 10.00 渭 g / kg, and the average recovery rate was 66.6 ~ 114.9 渭 g 路kg ~ (-1). The method is simple, sensitive and reproducible, and can be used for the synergistic determination of multicomponent mycotoxins in peanut oil and corn oil.
【作者单位】：北京农学院食品科学与工程学院;农产品有害微生物及农残安全检测与控制北京市重点实验室;国家食品安全风险评估中心卫生部食品安全风险评估重点实验室;中山大学公共卫生学院;
【基金】：国家自然科学基金青年科学基金项目(31301489) 北京市自然科学基金项目(7163235)
【分类号】：O657.63;TS227

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