在线亲和固相萃取-高效液相色谱-二极管阵列检测-四极杆飞行时间质谱快速筛选中药中α-葡萄糖苷酶结合成分

发布时间：2018-10-17 19:43

【摘要】：中药活性成分的快速分离分析一直是中药研究的重点和热点问题。该文建立了在线亲和固相萃取-高效液相色谱-二极管阵列检测-四极杆飞行时间质谱技术快速筛选中药中与α-葡萄糖苷酶有结合作用的活性成分的方法。四通阀和六通进样阀组成接口界面,收集亲和固相萃取柱中与α-葡萄糖苷酶有结合作用的活性成分,接着进入液相色谱系统进行分析鉴定。阳性对照品((+)-儿茶素)和阴性对照品(水杨酸)混合物筛选确定了方法的特异性和准确性。接着,以玉竹为研究对象,筛选出9种主要的α-葡萄糖苷酶抑制活性化合物,包括5种苯乙基肉桂酰胺类化合物,4种双氢高异黄酮类化合物。结果表明建立的方法简便、快速、特异性强,可用于任何复杂体系中α-葡萄糖苷酶结合活性成分的筛选。
[Abstract]:The rapid separation and analysis of active components of traditional Chinese medicine has been the focus and hot issue of traditional Chinese medicine research. An on-line affinity solid phase extraction (SPE)-high performance liquid chromatography (HPLC)-diode array detection-quadrupole time-of-flight mass spectrometry (TTOMS) was developed for rapid screening of 伪-glucosidase binding active components from Chinese traditional medicine. The four-way valve and the six-way sampling valve form the interface and collect the active components from the affinity solid-phase extraction column which bind to 伪 -glucosidase, and then enter the liquid chromatography system for analysis and identification. The specificity and accuracy of the method were determined by screening the mixture of ()-catechin and salicylic acid. Then, nine major 伪 -glucosidase inhibitory compounds were selected, including 5 phenylethyl cinnamamide compounds and 4 dihydrohyperisoflavone compounds. The results showed that the method was simple, rapid and specific, and could be used for the screening of 伪 -glucosidase binding active components in any complex system.
【作者单位】：中南大学化学化工学院;湖南省中医药研究院中药研究所;
【基金】：国家自然科学基金项目(21275163)~~
【分类号】：R284.1;O657.63

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