基于表面增强拉曼光谱测量尿液样本中血小板衍生生长因子-BB

发布时间：2018-10-19 11:38

【摘要】：通过测量不同低浓度血小板衍生生长因子-BB(PDGF-BB)水溶液的表面增强拉曼光谱(SERS),可以检测到质量分数低至10-8的PDGF-BB水溶液在拉曼频移为1509cm-1处的拉曼特征峰。通过检测行皮冠状动脉介入治疗术(PCI)患者(动脉粥样硬化程度较高)术前尿液样本与未行PCI患者(动脉粥样硬化程度较低)尿液样本SERS,并结合PDGF-BB的1509cm-1处拉曼特征峰可以发现,行PCI患者尿液样本的SERS中可以检测到PDGF-BB的拉曼特征峰,即PDGF-BB在尿液中的浓度与动脉粥样硬化程度相关。结果表明,SERS技术可以检测到极低浓度PDGF-BB水溶液的拉曼特征峰,尿液的SERS可以成为判断冠心病患者是否需要进行PCI的重要依据,因此SERS技术有望成为一种无损检测与筛查冠心病的临床诊断工具。
[Abstract]:Surface enhanced Raman spectroscopy (SERS),) of platelet-derived growth factor (PDGF-BB) aqueous solution with different concentrations can be used to detect the Raman characteristic of PDGF-BB aqueous solution with a mass fraction of 10 ~ (-8) to 1509cm-1. Urine samples from patients with (PCI) undergoing percutaneous coronary intervention (with high degree of atherosclerosis) and urine samples from patients without PCI (with lower degree of atherosclerosis) were detected by detecting the Raman characteristic peaks of 1509cm-1 in combination with PDGF-BB. Raman characteristic peaks of PDGF-BB can be detected in SERS of urine samples of patients with PCI, that is, the concentration of PDGF-BB in urine is correlated with the degree of atherosclerosis. The results showed that the Raman characteristic peaks of very low concentration PDGF-BB aqueous solution could be detected by SERS technique, and SERS in urine could be an important basis for judging the need for PCI in patients with coronary heart disease. Therefore, SERS technique is expected to be a clinical diagnostic tool for nondestructive detection and screening of coronary heart disease.
【作者单位】：上海理工大学能源与动力工程学院;上海市动力工程多相流动与传热重点实验室;上海交通大学医学院附属新华医院;
【基金】：国家自然科学基金(51676130,51306123) 上海市科委科研计划(13DZ2260900) 上海交通大学医学院转化医学创新基金(152H1004)
【分类号】：O657.37;R541.4

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