基于微流体脉冲驱动控制技术的电化学微流控芯片制备方法

发布时间：2018-10-21 20:55

【摘要】：基于微流体脉冲驱动控制技术搭建了电化学微流控芯片的制备系统。首先将纳米银墨水和甘油溶液分别微喷射到玻璃基底表面形成微电极图形和微流道液体阳模图形;然后分别进行烧结和聚二甲基硅氧烷(PDMS)模塑工艺制得微电极和微流道;最后将微电极和微流道键合形成电化学微流控芯片。研究了系统参量对液滴产生的影响以及液滴直径和重叠率对液滴成线的影响,制得的微电极最小线宽为45μm、厚度为2.2μm、电阻率为5.2μΩ·cm,制得的微流道最小线宽为35μm,流道表面光滑。采用制得的电化学微流控芯片进行了葡萄糖浓度的电化学流动检测。结果表明,葡萄糖溶液的浓度与响应电流具有较高的线性关系,可对一定浓度范围内的葡萄糖溶液进行定量检测。基于微流体脉冲驱动控制技术的电化学微流控芯片制备方法具有微喷射精度高、重复性好,制备系统结构简单、成本低廉等优点,可用于生化分析、生物传感器等领域的芯片制备。
[Abstract]:The fabrication system of electrochemical microfluidic chip is built based on microfluidic pulse drive control technology. Firstly, nano-silver ink and glycerol solution were microsprayed onto the surface of glass substrate to form microelectrode pattern and micro-channel liquid positive pattern, and then microelectrode and microchannel were prepared by sintering and polydimethylsiloxane (PDMS) molding process, respectively. Finally, an electrochemical microfluidic chip was formed by bonding the microelectrode and the microfluidic channel. The influence of the system parameters on the droplet and the influence of the droplet diameter and overlap rate on the droplet formation line were studied. The minimum linewidth and thickness of the microelectrode were 45 渭 m, 2.2 渭 m and 5.2 渭惟 cm, respectively. The minimum linewidth of the microchannel was 35 渭 m and the surface of the channel was smooth. Electrochemical flow detection of glucose concentration was carried out by electrochemical microfluidic chip. The results show that the concentration of glucose solution has a high linear relationship with the response current and can be quantitatively detected in a certain concentration range of glucose solution. The preparation method of electrochemical microfluidic chip based on micro-fluid pulse drive control technology has the advantages of high precision, good repeatability, simple structure and low cost, etc. It can be used in biochemical analysis. Chip fabrication in biosensor and other fields.
【作者单位】：南京理工大学机械工程学院;
【基金】：国家自然科学基金项目(No.51175268) 江苏省三维打印装备与制造重点实验室开放基金项目(No.BM2013006)资助~~
【分类号】：O629.1;O657.1

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