二氧化钛-AgNPs表面增强拉曼基底活性的研究
发布时间：2018-10-22 08:41
【摘要】：拉曼散射效应信号强度弱是拉曼散射本身固有的特性,因而导致检测灵敏度相应较低,在微量和痕量分析时发生困难。表面增强拉曼光谱技术能有效地克服这个弱点,使拉曼光谱技术的应用扩大到更为广泛的领域。尤其在生物医学领域,在过去的20年以来,拉曼光谱技术显示了很宽阔的应用前景,为医学工作者在解释疾病进展和病原学,以及在改善临床诊断和治疗方面都能提供很有价值的信息。本文除对拉曼光谱技术的基础知识进行相关介绍外,着重介绍了二氧化钛-AgNPs表面增强拉曼基底的制备及其活性的研究,采用532nm、632.8nm和785nm三种不同波长激光束,检测罗丹明6g(R6g)和健康人血清两种样品的表面增强拉曼光谱(SERSp),并验证不同波长激光激励对SERSp指纹谱的影响。结果表明：当提拉速度达到200mm/min时,二氧化钛薄膜光催化活性不再增加,影响基底活性的主要因素为粒子间距；440度-600度范围内,二氧化钛晶型均为锐钛矿结构；膜厚、温度及紫外灯照射时间共同影响银粒子生长,200mm/min提拉速度、520℃煅烧温度及紫外光照射80 min条件下获得的二氧化钛-AgNPs基底具有较好的拉曼增强效果；不同波长激励对SERSp各谱线的增强有明显的影响,在532nm波长激励的SERSp中,大波数的谱峰增强因子明显高于小波数的谱峰,在785nm波长激励的SERSp中又正好相反,而在632.8nm波长激励的SERSp中,大、小波数谱峰增强因子比较均一。不同波长激励样品SERSp指纹谱有明显的差异。最后介绍了该基底在肺癌筛查中的应用,初步结果表明,利用532nm波长激发光及支持向量机的分类方法,肺癌筛查正确率达89%,说明该方法具有进一步研究及实用的价值。
[Abstract]:The weak intensity of Raman scattering signal is the inherent characteristic of Raman scattering, which leads to low detection sensitivity and difficulty in trace and trace analysis. Surface-enhanced Raman spectroscopy can effectively overcome this weakness and expand the application of Raman spectroscopy to a wider range of fields. Especially in the field of biomedicine, over the past 20 years, Raman spectroscopy has shown a broad application prospect for medical workers in explaining disease progress and etiology, And can provide valuable information in improving clinical diagnosis and treatment. In addition to the introduction of the basic knowledge of Raman spectroscopy, the preparation and activity of TIO _ 2 AgNPs surface-enhanced Raman substrate are introduced in this paper. The laser beams of 532nm and 632.8 nm and 785nm are used. The surface enhanced Raman spectra (SERSp),) of Rhodamine 6g (R6g) and healthy serum samples were determined and the effects of laser excitation at different wavelengths on SERSp fingerprints were verified. The results show that the photocatalytic activity of TIO _ 2 films does not increase when the Czochralski rate reaches 200mm/min, and the main factors affecting the substrate activity are the particle spacing, the crystalline form of TIO _ 2 is anatase structure and the film thickness is in the range of 440 ~ 600 degrees. The growth of silver particles was affected by temperature and irradiation time of UV lamp. The 200mm/min Czochralski rate, calcination temperature at 520 鈩，
本文编号：2286671
[Abstract]:The weak intensity of Raman scattering signal is the inherent characteristic of Raman scattering, which leads to low detection sensitivity and difficulty in trace and trace analysis. Surface-enhanced Raman spectroscopy can effectively overcome this weakness and expand the application of Raman spectroscopy to a wider range of fields. Especially in the field of biomedicine, over the past 20 years, Raman spectroscopy has shown a broad application prospect for medical workers in explaining disease progress and etiology, And can provide valuable information in improving clinical diagnosis and treatment. In addition to the introduction of the basic knowledge of Raman spectroscopy, the preparation and activity of TIO _ 2 AgNPs surface-enhanced Raman substrate are introduced in this paper. The laser beams of 532nm and 632.8 nm and 785nm are used. The surface enhanced Raman spectra (SERSp),) of Rhodamine 6g (R6g) and healthy serum samples were determined and the effects of laser excitation at different wavelengths on SERSp fingerprints were verified. The results show that the photocatalytic activity of TIO _ 2 films does not increase when the Czochralski rate reaches 200mm/min, and the main factors affecting the substrate activity are the particle spacing, the crystalline form of TIO _ 2 is anatase structure and the film thickness is in the range of 440 ~ 600 degrees. The growth of silver particles was affected by temperature and irradiation time of UV lamp. The 200mm/min Czochralski rate, calcination temperature at 520 鈩，
本文编号：2286671
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