辣木叶的HPLC指纹图谱
发布时间：2018-10-24 07:43
【摘要】：目的:通过建立辣木叶的HPLC指纹图谱,评价不同产地辣木叶质量的差异,为辣木质量控制和评价提供科学依据,推动辣木的开发与利用。方法:采用HPLC法,Diamonsil Plus C_(18)色谱柱(4.6 mm×250 mm,5μm),流动相乙腈(A)-0.05%磷酸溶液(B)(0~70 min,5%~30%A;70~75 min,30%~100%A),流速1.0 mL·min~(-1),检测波长230 nm,柱温30℃。测定5个不同产地12批辣木叶的指纹图谱,并采用国家药典委员会颁布的"中药色谱指纹图谱相似度评价系统"(2004 A)和SPSS 21.0统计软件中的聚类分析对指纹图谱进行相似度比较。结果:建立辣木叶指纹图谱共有模式,标定14个指纹图谱共有峰,其中3个成分峰分别为没食子酸、芦丁、槲皮苷,为其有效成分。12批辣木叶中9批的相似度0.90,说明不同产地及同一产地不同地区辣木叶指纹图谱既有共性又存在一定差异。结论:建立的方法精密度、重复性和稳定性较好,可为辣木叶的鉴别和质量评价提供参考。
[Abstract]:Objective: to establish the HPLC fingerprint to evaluate the quality difference of the leaves from different producing areas, to provide the scientific basis for the quality control and evaluation of spicy wood, and to promote the development and utilization of spicy wood. Methods:, Diamonsil Plus C _ (18) column (4.6 mm 脳 250 mm,5 渭 m), mobile phase acetonitrile (A) -0.05% phosphoric acid solution (B) (070 min,5%~30%A;70~75 min,30%~100%A) was used by HPLC method. The flow rate was 1.0 mL min~ (-1). The detection wavelength was 230 nm, at 30 鈩，
本文编号：2290759
[Abstract]:Objective: to establish the HPLC fingerprint to evaluate the quality difference of the leaves from different producing areas, to provide the scientific basis for the quality control and evaluation of spicy wood, and to promote the development and utilization of spicy wood. Methods:, Diamonsil Plus C _ (18) column (4.6 mm 脳 250 mm,5 渭 m), mobile phase acetonitrile (A) -0.05% phosphoric acid solution (B) (070 min,5%~30%A;70~75 min,30%~100%A) was used by HPLC method. The flow rate was 1.0 mL min~ (-1). The detection wavelength was 230 nm, at 30 鈩，
本文编号：2290759
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