高效液相色谱法测定大豆中六种异黄酮含量方法的改进
发布时间：2018-10-24 13:06
【摘要】：为探索大豆异黄酮乙醇提取液的高效液相色谱检测方法,本研究按照改进后的色谱条件对分析方法学进行了评价。确定的色谱条件如下,流动相为0.1%乙酸-水溶液和0.1%乙酸-乙腈溶液,梯度洗脱;柱温30℃;检测波长260 nm;流速1.0 m L/min。六种大豆异黄酮线性范围良好,决定系数R20.9993,回收率范围99.62%~114.00%,相对标准偏差RSD范围0.96%~7.99%。本试验改进的检测方法线性关系、精密度、稳定性、准确度、灵敏度均较好,满足分析方法学评价的要求及大豆中六种异黄酮单体检测的要求,为改进大豆中异黄酮的检测方法提供了参考。
[Abstract]:In order to explore the determination method of soybean isoflavone ethanol extract by high performance liquid chromatography (HPLC), the analytical methodology was evaluated according to the improved chromatographic conditions. The chromatographic conditions are as follows: mobile phase: 0.1% acetic acid-aqueous solution and 0.1% acetic acid-acetonitrile solution, gradient elution, column temperature 30 鈩，
本文编号：2291500
[Abstract]:In order to explore the determination method of soybean isoflavone ethanol extract by high performance liquid chromatography (HPLC), the analytical methodology was evaluated according to the improved chromatographic conditions. The chromatographic conditions are as follows: mobile phase: 0.1% acetic acid-aqueous solution and 0.1% acetic acid-acetonitrile solution, gradient elution, column temperature 30 鈩，
本文编号：2291500
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