宁夏产柴胡药材与其对照药材的HPLC指纹图谱及近红外光谱比较

发布时间：2018-10-31 21:26

【摘要】：目的:通过宁夏13批柴胡药材与对照药材的HPLC指纹图谱和近红外图谱研究,对宁夏柴胡药材进行质量评价。方法:分别建立HPLC指纹图谱和近红外光谱,比较13批柴胡药材与对照药材HPLC和近红外光谱的相似度,并分别对13批柴胡药材和对照药材的共有峰面积和近红外吸收进行配对样本t检验。结果:13批柴胡药材与对照药材色谱指纹图谱和近红外图谱的相似度分别0.901和0.982,结合13批柴胡药材与对照药材共有峰面积(近红外为对应点吸收)的配对样本t检验验证,均无显著差异,验证了13批柴胡药材与对照药材质量的一致性较高。结论:本研究基于色谱指纹图谱与近红外图谱,并结合配对样本t检验对分析结果进行验证,认为13批宁夏产柴胡药材与对照药材质量无显著差异。研究采用两种综合性较强的检测手段,避免了分析中因指纹图谱相似度阈值限定不明确而引起的误判,为分析结果提供了可靠的依据,为完善中药材质量控制方法提供了新的思路。
[Abstract]:Objective: to evaluate the quality of Radix Bupleurum chinensis by HPLC fingerprint and near infrared spectrum of 13 batches of Radix Bupleurum in Ningxia. Methods: HPLC fingerprints and near infrared spectra were established, and the similarity of 13 batches of Bupleurum chinense with HPLC and NIR spectra were compared. The peak-area and near-infrared absorption of 13 batches of Bupleurum chinense were tested. Results: the similarity of 13 batches of Radix Bupleurum with chromatographic fingerprint and near infrared spectrum was 0.901 and 0.982, respectively. There was no significant difference between 13 batches of Bupleurum chinense and 13 batches of Bupleurum chinense. The quality consistency between the 13 batches of Bupleurum chinense and the control was higher than that of the control. Conclusion: this study was based on chromatographic fingerprint and near infrared spectrum, and combined with paired samples t test to verify the analysis results. It was concluded that there was no significant difference in the quality of 13 batches of Radix Bupleuri produced in Ningxia and the control. Two comprehensive detection methods are adopted to avoid the misjudgment caused by the uncertainty of the threshold of similarity in fingerprint analysis, which provides a reliable basis for the analysis results. It provides a new way to improve the quality control of traditional Chinese medicine.
【作者单位】：宁夏医科大学药学院;宁夏回族自治区药品检验所;宁夏回药现代化工程技术研究中心宁夏医科大学回医药现代化省部共建教育部重点实验室;
【基金】：宁夏科技支撑计划项目(2015BY032)
【分类号】：R284.1;O657

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