基于超高效液相色谱-四级杆串联飞行时间质谱联用法的牛奶质量控制和掺假鉴别
发布时间：2018-11-02 07:04
【摘要】：目的建立基于超高效液相色谱-四级杆串联飞行时间质谱联用结合模式识别分析法对牛奶进行质量控制和掺假物质鉴别。方法样品经高速离心除脂肪,乙腈除蛋白后,取上清,经ACQUITY UPLCTM BEH C18柱分离,QTOF MS/MS检测,PCA和PLS-DA对所获数据进行分类分析。结果牛奶和掺入不同物质的掺假牛奶在BPC图谱和PCA与PLS-DA得分图上可以有很好的区分,能准确筛检出差异物质或掺假物质。结论 UPLC/Q-TOF-MS/MS结合模式识别分析法可用于牛奶的质量控制和掺假鉴别。
[Abstract]:Objective to establish a method of quality control and identification of adulterated substances in milk based on ultra high performance liquid chromatography (HPLC)-series time of flight mass spectrometry (TTOMS). Methods the samples were separated by high speed centrifugation and protein removal by acetonitrile. The samples were separated by ACQUITY UPLCTM BEH C18 column and detected by QTOF MS/MS. The obtained data were classified and analyzed by PCA and PLS-DA. Results there was a good distinction between milk and adulterated milk on BPC map, PCA and PLS-DA score chart, and the difference substance or adulterated substance could be detected accurately. Conclusion UPLC/Q-TOF-MS/MS combined with pattern recognition analysis can be used for quality control and adulteration identification of milk.
【作者单位】： 齐齐哈尔医学院公共卫生学院;哈尔滨医科大学公共卫生学院;
【基金】：齐齐哈尔医学院科研基金项目(No.QY2013Q-08);齐齐哈尔医学院科研基金项目(No.QY2013Q-11)
【分类号】：TS252.7;O657.63
，
本文编号：2305298
[Abstract]:Objective to establish a method of quality control and identification of adulterated substances in milk based on ultra high performance liquid chromatography (HPLC)-series time of flight mass spectrometry (TTOMS). Methods the samples were separated by high speed centrifugation and protein removal by acetonitrile. The samples were separated by ACQUITY UPLCTM BEH C18 column and detected by QTOF MS/MS. The obtained data were classified and analyzed by PCA and PLS-DA. Results there was a good distinction between milk and adulterated milk on BPC map, PCA and PLS-DA score chart, and the difference substance or adulterated substance could be detected accurately. Conclusion UPLC/Q-TOF-MS/MS combined with pattern recognition analysis can be used for quality control and adulteration identification of milk.
【作者单位】： 齐齐哈尔医学院公共卫生学院;哈尔滨医科大学公共卫生学院;
【基金】：齐齐哈尔医学院科研基金项目(No.QY2013Q-08);齐齐哈尔医学院科研基金项目(No.QY2013Q-11)
【分类号】：TS252.7;O657.63
，
本文编号：2305298
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