吴茱萸UPLC指纹图谱的建立及质量评价
发布时间：2018-11-03 18:10
【摘要】：目的:建立吴茱萸超高效液相色谱(UPLC)指纹图谱分析方法,对不同品种、不同产地吴茱萸药材的质量进行较全面的评价。方法:采用ACQUITY UPLC BEH C18色谱柱(2.1 mm×100 mm,1.7μm),以乙腈-0.1%甲酸水为流动相进行梯度洗脱,检测波长300,215 nm,流速0.4 m L·min~(-1),柱温35℃。采用相似度评价、聚类分析及偏最小二乘法判别分析对37批样品的指纹图谱进行分析。结果:建立了吴茱萸药材的UPLC指纹图谱及共有模式,标定了34个共有峰,采用对照品比对,指认出17个共有峰。37批样品的相似度在0.703~0.973。不同来源的吴茱萸药材指纹图谱轮廓基本一致,化学成分的相对含量在较大范围内波动。聚类分析和偏最小二乘判别分析结果表明,化学成分的相对含量与药材品种、产地没有明显的相关性,而与药材的千粒重在一定程度上相关;吴茱萸次碱、吴茱萸碱、新绿原酸、绿原酸、吴茱萸卡品碱、柠檬苦素是差异较大的6个成分。结论:该方法简便、快速,可有效、全面地用于吴茱萸药材的质量评价;控制吴茱萸药材质量的关键是把握"近成熟"这一采收时间环节。
[Abstract]:Objective: to establish a (UPLC) fingerprint analysis method for Evodia rutaecarpa and evaluate the quality of Evodia rutaecarpa from different varieties and different producing areas. Methods: a ACQUITY UPLC BEH C18 column (2.1 mm 脳 100 mm,1.7 渭 m),) was used for gradient elution with acetonitrile-0.1% formic acid water as mobile phase. The detection wavelength was 300215 nm, and the flow rate was 0.4 mL min~ (-1). The column temperature was 35 鈩，
本文编号：2308557
[Abstract]:Objective: to establish a (UPLC) fingerprint analysis method for Evodia rutaecarpa and evaluate the quality of Evodia rutaecarpa from different varieties and different producing areas. Methods: a ACQUITY UPLC BEH C18 column (2.1 mm 脳 100 mm,1.7 渭 m),) was used for gradient elution with acetonitrile-0.1% formic acid water as mobile phase. The detection wavelength was 300215 nm, and the flow rate was 0.4 mL min~ (-1). The column temperature was 35 鈩，
本文编号：2308557
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