蛇莓中极性成分的研究

发布时间：2018-11-04 13:55

【摘要】：蛇莓(Duchesnea indica(Andr.)Focke),又名地莓等,为蔷薇科多年生草本植物,作为传统的中药材在《名医别录》和《本草纲目》中均有记载。蛇莓分布广泛,在中国尤其是长江流域资源丰富,在印度,欧洲以及美洲也有分布。蛇莓的用途广泛,药用有清热解毒、散瘀消肿、凉血止血、治疗蛇虫咬伤等功效。本文总结了近年来蛇莓的化学成分和药理作用的研究成果,发现在蛇莓的DPPH自由基清除活性和酪氨酸酶抑制活性方面的报道较少,因此,本文选用这两种活性为导向,采用MCI柱层析,大孔树脂柱层析以及ODS-A反相柱层析和高效液相色谱等分离手段,从长白山区蔷薇科植物蛇莓中分离得到13个化合物,并对其中6个极性化合物的分子结构进行结构鉴定,它们分别为3-O-β-D-glucopyranoxy-6-hydroxy-p-cymene(1),没食子酸甲酯(Methyl 3,4,5-trih-ydroxybenzoate)(2),黑色五味子单体苷(Isolariciresinol-2α-O-β-D-xyloside)(6),牡荆素(Apigenin-8-C-glucoside)(9),1-羟基松脂醇-1-β-D-葡萄糖苷[(+)-1-Hydroxysyringaresinol-1-β-D-glucoside](10),(6R,9R)-3-氧代-α-紫罗兰醇-β-D-葡萄糖苷[(6R,9R)-3-Oxo-α-ionol-β-D-glucopyranoside](13)。对分离并鉴定结构的化合物分别进行了 DPPH自由基清除和酪氨酸酶活性抑制实验,结果显示,所有化合物的DPPH自由基清除率和酪氨酸酶抑制率均在90%以上,其中化合物10,13的DPPH自由基清除活性IC50值分别为2.23μg/mL、2.07μg/mL,小于阳性参展组维生素C。化合物2,13的酪氨酸酶抑制率高于参照组曲酸且化合物13的酪氨酸酶抑制活性IC50值为3.3 μg/mL。
[Abstract]:(Duchesnea indica (Andr.) Focke), is a perennial herb of Rosaceae. As a traditional Chinese herbal medicine, it is recorded in the famous Medical Records and Compendium of Materia Medica (Compendium of Materia Medica). It is widely distributed in China, especially in the Yangtze River Basin, and also in India, Europe and America. Snake berries are widely used in medicine, such as clearing heat and detoxification, dispersing blood stasis and swelling, cooling blood and hemostasis, treating snakebug bite and so on. In this paper, the chemical constituents and pharmacological effects of Raspberry were summarized. It was found that there were few reports on DPPH radical scavenging activity and tyrosinase inhibitory activity. By means of MCI column chromatography, macroporous resin column chromatography, ODS-A reversed phase chromatography and high performance liquid chromatography, 13 compounds were isolated from Rosaceae plants in Changbai Mountain area. The molecular structures of the six polar compounds were identified as 3-O- 尾-D-glucopyranoxy-6-hydroxy-p-cymene (1), Methyl _ 3N _ 4 (5-trih-ydroxybenzoate) (2). Black Schisandrin (Isolariciresinol-2 伪 -O- 尾 D-xyloside) (6), Apigenin-8-C-glucoside (9), 1- 尾 -D-glucoside [() -1-Hydroxysyringaresinol-1- 尾-D-glucoside] (10), (6RN9R) -3-oxo- 伪 -ionol-尾 -D-glucoside [(6R,) 9R) -3-Oxo- 伪-ionol- 尾-D-glucopyranoside] (13). The DPPH radical scavenging and tyrosinase activity inhibition experiments were carried out on the isolated and identified compounds. The results showed that the DPPH radical scavenging rate and tyrosinase inhibition rate of all compounds were above 90%. The free radical scavenging activity IC50 of compound 10C13 was 2.23 渭 g / mL and 2.07 渭 g / mL, respectively, which was lower than that of positive exhibitors. The inhibitory rate of tyrosinase of compound 2C13 was higher than that of control group, and the inhibitory activity of tyrosinase of compound 13 was 3.3 渭 g / mL.
【学位授予单位】：延边大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：R284;O652.6
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本文编号：2310018