溶剂诱导相变萃取-UPC~2快速测定中成药及原料药中的香豆素
发布时间:2018-11-05 20:37
【摘要】:使用溶剂诱导相变萃取对中成药及原料药进行预处理,建立了中成药中香豆素的超高效合相色谱(UPC2)检测方法。样品经粉碎(60目)、乙腈-水(7∶3,体积比)提取,二氯甲烷诱导分相后,使用UPC2对样品中的香豆素进行检测。以3倍信噪比(S/N)确定香豆素的检出限为0.1 mg/L;线性范围为0.3~10.0mg/L;加标回收率为89.8%~102.3%;相对标准偏差为0.31%~1.0%。同时与UPLC进行比较,结果表明该方法的选择性高,检测成本低,且分析时间仅为UPLC的1/4。该研究为高通量检测香豆素提供了一种全新的前处理及检测方法。
[Abstract]:Solvent-induced phase change extraction (SPE) was used to pretreat Chinese patent medicine and raw material, and a method for the determination of coumarin in Chinese patent medicine by ultra-high performance phase chromatography (UPC2) was established. The samples were pulverized (60 mesh), acetonitrile-water (7: 3, volume ratio) was extracted, dichloromethane induced phase separation, and coumarin in the sample was determined by UPC2. The detection limit of coumarin is 0. 1 mg/L;, the linear range is 0. 310. 0 mg / L, the recovery rate is 89. 8% and the relative standard deviation is 0. 31% and 1. 0%, respectively. Compared with UPLC, the results show that the method has high selectivity and low detection cost, and the analysis time is only 1 / 4 of that of UPLC. This study provides a new pretreatment and detection method for high-throughput coumarin detection.
【作者单位】: 甘肃出入境检验检疫局综合技术中心;甘肃省检验检疫科学技术研究院;
【分类号】:O657.7;TQ460.72
,
本文编号:2313380
[Abstract]:Solvent-induced phase change extraction (SPE) was used to pretreat Chinese patent medicine and raw material, and a method for the determination of coumarin in Chinese patent medicine by ultra-high performance phase chromatography (UPC2) was established. The samples were pulverized (60 mesh), acetonitrile-water (7: 3, volume ratio) was extracted, dichloromethane induced phase separation, and coumarin in the sample was determined by UPC2. The detection limit of coumarin is 0. 1 mg/L;, the linear range is 0. 310. 0 mg / L, the recovery rate is 89. 8% and the relative standard deviation is 0. 31% and 1. 0%, respectively. Compared with UPLC, the results show that the method has high selectivity and low detection cost, and the analysis time is only 1 / 4 of that of UPLC. This study provides a new pretreatment and detection method for high-throughput coumarin detection.
【作者单位】: 甘肃出入境检验检疫局综合技术中心;甘肃省检验检疫科学技术研究院;
【分类号】:O657.7;TQ460.72
,
本文编号:2313380
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