柯里拉京与DNA相互作用的光谱研究
发布时间：2018-11-09 13:27
【摘要】：在p H 7.4的生理条件下,以溴化乙锭(EB)作为荧光探针,利用荧光光谱、紫外-可见吸收光谱、共振散射光谱法结合盐效应和DNA熔点(T_m)实验研究了柯里拉京(Cor)与小牛胸腺DNA分子之间的相互作用机制。实验结果表明,Cor静态猝灭DNA-EB体系的荧光。Cor与DNA作用后,其特征吸收峰强度发生减色效应;与DNA作用导致Cor在480.5 nm处的共振散射峰增强,并在330.2 nm处出现新共振散射峰。盐效应对Cor与DNA分子相互作用的影响较小。与Cor作用引起DNA的T_m值升高5.5℃。由此推断,Cor与DNA相互作用的主要方式为嵌插,两者间形成了超分子体系。通过计算获得Cor与DNA间结合常数(K_A)为5.82×10~3L/mol(298 K)、2.47×10~4L/mol(310 K),它们之间的作用为熵驱动的自发、吸热过程,疏水作用力是主要的非共价作用方式。
[Abstract]:Under physiological conditions of pH 7.4, ethidium bromide (EB) was used as a fluorescence probe. The mechanism of interaction between Coriragin (Cor) and calf thymus DNA molecule was studied by resonance scattering spectroscopy combined with salt effect and DNA melting point (T _ m) experiment. The experimental results show that the fluorescence of DNA-EB system is quenched by Cor statically, and the characteristic absorption peak intensity of Cor decreases after the interaction of Cor with DNA. The interaction with DNA leads to the enhancement of the resonance scattering peak of Cor at 480.5 nm and the appearance of new resonance scattering peak at 330.2 nm. The salt effect has little effect on the interaction between Cor and DNA molecules. Interaction with Cor resulted in a 5.5 鈩，
本文编号：2320539
[Abstract]:Under physiological conditions of pH 7.4, ethidium bromide (EB) was used as a fluorescence probe. The mechanism of interaction between Coriragin (Cor) and calf thymus DNA molecule was studied by resonance scattering spectroscopy combined with salt effect and DNA melting point (T _ m) experiment. The experimental results show that the fluorescence of DNA-EB system is quenched by Cor statically, and the characteristic absorption peak intensity of Cor decreases after the interaction of Cor with DNA. The interaction with DNA leads to the enhancement of the resonance scattering peak of Cor at 480.5 nm and the appearance of new resonance scattering peak at 330.2 nm. The salt effect has little effect on the interaction between Cor and DNA molecules. Interaction with Cor resulted in a 5.5 鈩，
本文编号：2320539
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