HPLC法同时测定风湿关节炎片中4种成分的含量

发布时间：2018-11-11 19:06

【摘要】：目的:建立同时测定风湿关节炎片中盐酸麻黄碱、盐酸伪麻黄碱、马钱子碱、士的宁含量的方法。方法:采用高效液相色谱法。色谱柱为XTerra-C_(18),流动相为乙腈-0.01 mol/L庚烷磺酸钠与0.01 mol/L磷酸二氢钾的等量混合溶液(用磷酸调p H至2.8)(梯度洗脱),流速为1.0 ml/min,检测波长为206 nm(盐酸麻黄碱和盐酸伪麻黄碱)、260 nm(马钱子碱和士的宁),柱温为30℃,进样量为10μl。结果:盐酸伪麻黄碱、盐酸麻黄碱、马钱子碱和士的宁检测质量浓度线性范围分别为0.916~18.320 0μg/ml(r=0.999 6)、10.034~200.680 0μg/ml(r=0.999 7)、8.693~121.700 0μg/ml(r=0.999 9)、9.696~193.920 0μg/ml(r=0.999 9);定量限分别为0.03、0.07、0.04、0.03 ng,检测限分别为0.01、0.02、0.01、0.01 ng;精密度、稳定性、重复性试验的RSD2.0%;加样回收率分别为97.30%~99.79%(RSD=0.96%,n=6)、95.81%~99.36%(RSD=0.38%,n=6)、95.34%~99.93%(RSD=2.00%,n=6)、95.59%~99.80%(RSD=1.76%,n=6)。结论:该方法操作简便、结果准确,可用于同时测定风湿关节炎片中盐酸麻黄碱、盐酸伪麻黄碱、马钱子碱、士的宁的含量。
[Abstract]:Objective: to establish a method for simultaneous determination of ephedrine hydrochloride, pseudoephedrine hydrochloride, brucine and strychnine in rheumatoid arthritis tablets. Methods: high performance liquid chromatography was used. The chromatographic column was XTerra-C_ (18). The mobile phase consisted of acetonitrile-0.01 mol/L sodium heptane sulfonate and 0.01 mol/L potassium dihydrogen phosphate (adjusted by phosphoric acid to 2.8) (gradient elution). The flow rate was 1. 0 ml/min,. The detection wavelength was 206 nm (ephedrine hydrochloride and pseudoephedrine hydrochloride), 260 nm (brucine and strychnine), the column temperature was 30 鈩，

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