酸枣仁黄酮HPLC指纹图谱建立及2种成分测定
发布时间：2018-11-14 09:54
【摘要】：目的建立酸枣仁Ziziphi spinosae Semen黄酮HPLC指纹图谱,并测定其中2种成分的含有量。方法以斯皮诺素为参照峰,建立10批样品的HPLC指纹图谱。酸枣仁黄酮甲醇提取物的分析采用Agilent TC-C_(18)色谱柱(4.6 mm×250 mm,5μm);流动相乙腈-0.1%醋酸,梯度洗脱;体积流量1 m L/min;柱温30℃;检测波长280 nm。结果 HPLC指纹图谱中有10个共有峰,并鉴定出其中8个(维采宁-Ⅱ、牡荆素葡萄糖苷、异斯皮诺素、斯皮诺素、6'''-pyridyloylspinosin、6'''-对羟基苯甲酰斯皮诺素、6'''-阿魏酰斯皮诺素、6'''-对香豆酰斯皮诺素)。斯皮诺素和6'''-阿魏酰斯皮诺素分别在15.00~40.00μg和5.00~14.00μg范围内线性关系良好,平均加样回收率(RSD)分别为100.5%(1.6%)和100.4%(1.6%)。结论该方法准确、简单、可靠,可用于酸枣仁黄酮的质量控制。
[Abstract]:Objective to establish the HPLC fingerprint of Ziziphi spinosae Semen flavonoids from jujube kernel and determine the content of two components. Methods the HPLC fingerprint of 10 batches of samples was established with spinosin as the reference peak. Analysis of flavonoid methanol extract from Ziziphus jujube seed by Agilent TC-C_ (18) column (4.6 mm 脳 250 mm,5 渭 m);) mobile phase acetonitrile-0.1% acetic acid, gradient elution; volume flow rate 1 mL / min; column temperature 30 鈩，
本文编号：2330834
[Abstract]:Objective to establish the HPLC fingerprint of Ziziphi spinosae Semen flavonoids from jujube kernel and determine the content of two components. Methods the HPLC fingerprint of 10 batches of samples was established with spinosin as the reference peak. Analysis of flavonoid methanol extract from Ziziphus jujube seed by Agilent TC-C_ (18) column (4.6 mm 脳 250 mm,5 渭 m);) mobile phase acetonitrile-0.1% acetic acid, gradient elution; volume flow rate 1 mL / min; column temperature 30 鈩，
本文编号：2330834
本文链接：https://www.wllwen.com/kejilunwen/huaxue/2330834.html
教材专著
