新型纳米材料的制备及其在细胞色素C检测中的应用
发布时间：2018-11-16 18:14
【摘要】：自上世纪90年代以来,纳米技术获得了持续发展。由于其独特的物理和化学性质,纳米材料得到了广泛的研究和应用。新型纳米材料与荧光、电化学技术的结合,为分析化学带来了更有效的分析方法。细胞色素C是细胞凋亡的重要信息物质之一。发展简单、快速的细胞色素C的检测方法对于临床检测和生物学研究具有重要意义。在本论文中,研究了将纳米技术与光电化学、荧光分析技术相结合,开发细胞色素C的快速检测方法。具体包括如下三个方面的工作：(1)CdS/ZnIn2S4/TiO2三维异质结构的制备及其光电性能的研究。以Ti02纳米管阵列为基底,通过简单水热法和连续离子层吸附和反应法,制备三维CdS/ZnIn2S4/TiO2异质结构。在AM1.5G辐射下,CdS/ZnIn2S4/TiO2显示了优良的光电性能,光电流达到4.3 mA/cm2,光电转换效率为2%。本研究为更有效地利用太阳光光谱提供了一种新型三维异质结构体系的设计路线。与此同时,本研究对材料进行了光催化降解性能测试,值得注意的是,在持续90 min的光照后,100%的二氯苯氧基乙酸(2,4-D)被移除,而使用ZnIn2S4/TiO2和TiO2纳米管阵列在同一条件相同时间内分别只能达到72.3%和39.1%的去除率。稳定性实验证明材料具有良好的化学稳定性。此外,本课题详细地研究了该材料进行光催化降解的机制。(2)基于Au/CdS/ZnIn2S4/TiO2复合电极材料的无标记细胞色素C光电感器的研究。通过恒电位电化学沉积法将金纳米颗粒沉积到CdS/ZnIn2S4/TiO2复合电极表面,获得性能优异的Au/CdS/ZnIn2S4/TiO2纳米复合材料,并将其作为光电极：以巯基修饰的细胞色素C特异性适配体为识别物质与电极结合,构建无标记光电适配体传感器实现对细胞色素C的定量检测。检测原理：通过检测适配体-细胞色素C相结合,导致的光电流变化情况,间接测定细胞色素C的浓度。本方法中,利用对细胞色素C具有高特异性识别性能的适配体和高灵敏电化学检测方法的结合,使传感体系具备高度的选择性和相对较低的检测限(1.0pM)。此外,进一步的实验数据证明了该传感器具有良好的重现性和稳定性。(3)基于VS2纳米片的新型生物传感器快速荧光检测细胞色素C的研究。由于细胞色素C本身不具有荧光,所以无法直接通过检测其自身荧光来进行定性或定量。本研究通过间接荧光恢复的方法对其进行测定。通过简单溶液方法合成具有强荧光猝灭能力的VS2纳米片,并将其作为传感响应平台；末端用荧光染料羧基荧光素标记的能与细胞色素C特异性结合的适配体作为识别探针。VS2纳米片能有效地吸附探针并猝灭探针末端荧光团的荧光。然而,当荧光探针先与细胞色素C进行孵育,再与VS2纳米片溶液混合,探针的荧光将不会被猝灭。由于所用适配体的高特异性能,传感器对细胞色素C显示出了高度的选择性和灵敏度,其线性范围为0.75nM-50μM,检测限为0.5 nM,同一检测条件下对生物体内其他干扰物基本无检出。
[Abstract]:Nanotechnology has been continuously developing since the 1990s. Due to its unique physical and chemical properties, the nano-materials have been widely studied and applied. The combination of new nano-materials with fluorescence and electrochemical technology has brought more effective analytical methods for analytical chemistry. Cytochrome C is one of the important information materials of cell apoptosis. The development of simple and rapid detection of cytochrome C is of great significance for clinical detection and biological research. In this paper, the method of rapid detection of cytochrome C was developed by combining nano-technology with photochemistry and fluorescence analysis. The method comprises the following three aspects: (1) the preparation of the CdS/ ZnIn2S4/ TiO2 three-dimensional heterostructure and the research on the photoelectric property thereof. Three-dimensional CdS/ ZnIn2S4/ TiO2 heterostructures were prepared by simple hydrothermal method and continuous ion-layer adsorption and reaction. CdS/ ZnIn2S4/ TiO2 showed excellent photoelectric properties under the radiation of AM1.5G, and the photocurrent reached 4. 3mA/ cm2 and the photoelectric conversion efficiency was 2%. The present study provides a new design route of a new three-dimensional heterostructure system for the more efficient use of the solar spectrum. At the same time, the present study tested the photocatalytic degradation of the material, noting that 100% of the dichlorophenoxyacetic acid (2, 4-D) was removed after 90 min of illumination, The use of ZnIn2S4/ TiO2 and TiO2 nanotube arrays can only reach 72.3% and 39.1% of the removal rate in the same time. The stability experiment proves that the material has good chemical stability. In addition, the mechanism of photocatalytic degradation of the material was studied in detail in this paper. (2) The study of the non-labeled cytochrome C photoinductor based on the Au/ CdS/ ZnIn2S4/ TiO2 composite electrode material. the gold nanoparticles are deposited on the surface of the CdS/ ZnIn2S4/ TiO2 composite electrode by a constant potential electrochemical deposition method, and the Au/ CdS/ ZnIn2S4/ TiO2 nano composite material with excellent performance is obtained, and the quantitative detection of the cytochrome C is realized by constructing a non-labeled photoelectric aptamer sensor. the detection principle: the concentration of the cytochrome c is indirectly determined by detecting the change of the photocurrent caused by the combination of the aptamer-cytochrome c. In the method, the sensor system is provided with a high selectivity and a relatively low detection limit (1,0pM) by combining an aptamer with high specificity identification performance and a high-sensitivity electrochemical detection method for cytochrome C. Furthermore, further experimental data demonstrate that the sensor has good reproducibility and stability. (3) Study on the rapid fluorescence detection of cytochrome C by novel biosensor based on VS2 nanosheet. Since the cytochrome C itself does not have fluorescence, it is not possible to make a qualitative or quantitative determination by detecting its own fluorescence. This study was determined by means of indirect fluorescence recovery. The VS2 nanosheet with strong fluorescence quenching capacity is synthesized by a simple solution method, and is used as a sensing response platform, and the terminal is used as an identification probe with a fluorescent dye-based fluorescein-labeled aptamer capable of specifically binding to the cytochrome C. The VS2 nanosheet can effectively adsorb the probe and wipe out the fluorescence of the fluorophore at the end of the probe. However, when the fluorescent probe is first incubated with cytochrome C and then mixed with the VS2 nanosheet solution, the fluorescence of the probe will not be inactivated. Due to the high specific energy of the aptamer used, the sensor showed a high degree of selectivity and sensitivity to the cytochrome C, with a linear range of 0.75nM to 500.mu. M, with a detection limit of 0.5 nM, and the other interfering substances in the organism were substantially free of detection under the same detection conditions.
【学位授予单位】：湖南大学
【学位级别】：硕士
【学位授予年份】：2016
【分类号】：TB383.1;O657.3
，
本文编号：2336259
[Abstract]:Nanotechnology has been continuously developing since the 1990s. Due to its unique physical and chemical properties, the nano-materials have been widely studied and applied. The combination of new nano-materials with fluorescence and electrochemical technology has brought more effective analytical methods for analytical chemistry. Cytochrome C is one of the important information materials of cell apoptosis. The development of simple and rapid detection of cytochrome C is of great significance for clinical detection and biological research. In this paper, the method of rapid detection of cytochrome C was developed by combining nano-technology with photochemistry and fluorescence analysis. The method comprises the following three aspects: (1) the preparation of the CdS/ ZnIn2S4/ TiO2 three-dimensional heterostructure and the research on the photoelectric property thereof. Three-dimensional CdS/ ZnIn2S4/ TiO2 heterostructures were prepared by simple hydrothermal method and continuous ion-layer adsorption and reaction. CdS/ ZnIn2S4/ TiO2 showed excellent photoelectric properties under the radiation of AM1.5G, and the photocurrent reached 4. 3mA/ cm2 and the photoelectric conversion efficiency was 2%. The present study provides a new design route of a new three-dimensional heterostructure system for the more efficient use of the solar spectrum. At the same time, the present study tested the photocatalytic degradation of the material, noting that 100% of the dichlorophenoxyacetic acid (2, 4-D) was removed after 90 min of illumination, The use of ZnIn2S4/ TiO2 and TiO2 nanotube arrays can only reach 72.3% and 39.1% of the removal rate in the same time. The stability experiment proves that the material has good chemical stability. In addition, the mechanism of photocatalytic degradation of the material was studied in detail in this paper. (2) The study of the non-labeled cytochrome C photoinductor based on the Au/ CdS/ ZnIn2S4/ TiO2 composite electrode material. the gold nanoparticles are deposited on the surface of the CdS/ ZnIn2S4/ TiO2 composite electrode by a constant potential electrochemical deposition method, and the Au/ CdS/ ZnIn2S4/ TiO2 nano composite material with excellent performance is obtained, and the quantitative detection of the cytochrome C is realized by constructing a non-labeled photoelectric aptamer sensor. the detection principle: the concentration of the cytochrome c is indirectly determined by detecting the change of the photocurrent caused by the combination of the aptamer-cytochrome c. In the method, the sensor system is provided with a high selectivity and a relatively low detection limit (1,0pM) by combining an aptamer with high specificity identification performance and a high-sensitivity electrochemical detection method for cytochrome C. Furthermore, further experimental data demonstrate that the sensor has good reproducibility and stability. (3) Study on the rapid fluorescence detection of cytochrome C by novel biosensor based on VS2 nanosheet. Since the cytochrome C itself does not have fluorescence, it is not possible to make a qualitative or quantitative determination by detecting its own fluorescence. This study was determined by means of indirect fluorescence recovery. The VS2 nanosheet with strong fluorescence quenching capacity is synthesized by a simple solution method, and is used as a sensing response platform, and the terminal is used as an identification probe with a fluorescent dye-based fluorescein-labeled aptamer capable of specifically binding to the cytochrome C. The VS2 nanosheet can effectively adsorb the probe and wipe out the fluorescence of the fluorophore at the end of the probe. However, when the fluorescent probe is first incubated with cytochrome C and then mixed with the VS2 nanosheet solution, the fluorescence of the probe will not be inactivated. Due to the high specific energy of the aptamer used, the sensor showed a high degree of selectivity and sensitivity to the cytochrome C, with a linear range of 0.75nM to 500.mu. M, with a detection limit of 0.5 nM, and the other interfering substances in the organism were substantially free of detection under the same detection conditions.
【学位授予单位】：湖南大学
【学位级别】：硕士
【学位授予年份】：2016
【分类号】：TB383.1;O657.3
，
本文编号：2336259
本文链接：https://www.wllwen.com/kejilunwen/huaxue/2336259.html
教材专著
