在线测定秦皮甲素和秦皮乙素水解反应速率常数的扫集-流动注射-芯片毛细管胶束电动色谱方法

发布时间：2018-11-19 15:32

【摘要】：建立了在线测定秦皮甲素和秦皮乙素水解反应速率常数的扫集-流动注射-胶束电动色谱新方法。该方法进样频率为12次/h,可在5 min内完全分离反应体系中的所有组分,30 min即可完成一个温度下的反应速率常数测定。由于该在线测定方法不需要终止水解反应,通过一次连续进样分析得到水解反应过程电泳谱图,从而可以获得水解反应过程中的一些信息。在最佳条件时,用0.1 mol/L KOH为催化剂,测得25,30,35,40和45℃时秦皮甲素的水解反应速率常数分别为3.65×10~(-2)/min、5.24×10~(-2)/min、7.12×10~(-2)/min、10.5×10~(-2)/min和16.3×10~(-2)/min,活化能为58.57 kJ/mol。用10 mmol/L KOH为催化剂,测得15,20,25,30和35℃时秦皮乙素的水解反应速率常数分别为2.26×10~(-2)/min,2.85×10~(-2)/min,3.55×10~(-2)/min,4.38×10~(-2)/min和5.29×10~(-2)/min,活化能为31.55 kJ/mol。
[Abstract]:A new scanning-flow injection-micellar electrokinetic chromatography method was established for the on-line determination of the rate constants of the hydrolysis reaction of chrysin A and B. The sampling frequency of this method is 12 times / h, and all components of the reaction system can be completely separated within 5 min, and the reaction rate constant can be determined at a temperature of 30 min. Because the on-line determination method does not need to terminate the hydrolysis reaction, the electrophoretic spectrum of the hydrolysis reaction process can be obtained by a continuous injection analysis, and some information in the hydrolysis reaction process can be obtained. Under the optimum conditions, the hydrolysis rate constants of cinnabinol at 25 鈩，

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