远志UPLC多指标成分的测定及指纹图谱研究

发布时间：2018-11-25 22:30

【摘要】：目的建立远志超高效液相色谱(UPLC)多成分测定方法及指纹图谱方法。方法采用UPLC法,以乙腈-0.1%甲酸水溶液梯度洗脱,体积流量0.4 m L/min,检测波长320 nm,测定24批远志药材中sibiricose A5、sibiricose A6、sibiricaxanthone B、glomeratose A、polygalaxanthone III、3,6′-disinapoyl sucrose 6种成分的量,并建立远志药材指纹图谱,采用超高效液相色谱-飞行时间质谱(UPLC-Q-TOF-MS~E)指认共有峰。结果不同产地远志中3,6′-disinapoyl sucrose与sibiricose A6量差异较大;建立的药材UPLC指纹图谱共标定37个共有峰,指认出其中33个峰,24批样品相似度在0.756~0.997。成分量及相似度综合分析结果表明不同地区远志药材质量存在一定差异。结论建立的测定结合指纹图谱分析方法可有效、全面地用于远志药材的质量评价。
[Abstract]:Objective to establish a multi-component determination method and fingerprint chromatographic method for (UPLC) of Radix Polygali by ultra-high-performance liquid chromatography. Methods UPLC method was used to determine sibiricose A5 sibiricose A6 sibiricanthone Bconglomeratose ApolyGalaxy III, in 24 batches of Radix Polygoni by gradient elution with acetonitrile-0.1% formic acid solution and volume flow rate of 0.4 mL / min. The detection wavelength was 320 nm,. The contents of six components of sucrose were determined, and the fingerprint of Radix Polygala was established, and the common peaks were identified by ultra-high performance liquid chromatography-time-of-flight mass spectrometry (UPLC-Q-TOF-MS~E). Results there was a significant difference in the amount of sucrose and sibiricose A6 between Radix Polygoni from different habitats, and there were 37 common peaks identified by the established UPLC fingerprint, of which 33 peaks were identified, and the similarity of 24 samples was 0.7560.997. The results of composition and similarity analysis showed that there were some differences in the quality of Radix Polygala in different regions. Conclusion the established method combined with fingerprint analysis can be used to evaluate the quality of Radix Polygala.
【作者单位】：中国药科大学天然药物活性组分与药效国家重点实验室;天士力控股集团有限公司;
【分类号】：R286.022

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