白蒿挥发油成分的测定及其抗氧化活性分析

发布时间：2018-11-28 21:05

【摘要】：采用乙醇浸提法提取生长期和花果期白蒿的挥发油,通过正交实验确定最佳提取条件为:提取3次、浸提90 min、对于生长期和花果期白蒿液料比分别为10 m L∶1 g和8 m L∶1 g。通过红外光谱和液相色谱-质谱联用分析了产物的主要成分,生长期白蒿挥发油的主要成分为桉树脑、樟脑、右旋龙脑、石竹烯、Cadina-1,4-diene、白菖烯、棕榈酸乙酯等;花果期白蒿挥发油的主要成分为樟脑、石竹烯、龙脑、甲酸橙花酯等。通过1,1-二苯基-2-三硝基苯肼(DPPH)自由基清除实验对产物的自由基清除活性进行了测定,生长期和花果期白蒿挥发油对0.05 mmol/L DPPH溶液的IC50值分别为0.40和1.66 mg/m L。研究结果表明,乙醇浸提的生长期和花果期白蒿的挥发油具有较好的抗氧化活性。
[Abstract]:The essential oil of Artemisia annua in growing period and flower and fruit period was extracted by ethanol extraction. The optimum extraction conditions were determined by orthogonal experiment: 3 times, 90 min,. For the growing period and flowering and fruit stage, the ratio of liquid to material of Artemisia annua was 10 mL: 1 g and 8 m L: 1 g, respectively. The main components of the volatile oil of Artemisia annua were analyzed by IR and LC-MS. The main components of volatile oil of Artemisia annua were eucalyptus, camphor, dextral borneol, caryophyllene, Cadina-1,4-diene, ethyl palmitate and so on. The main components of volatile oil of Artemisia annua in flower and fruit period are camphor, caryophyllene, borneol, orange-formate and so on. The scavenging activity of 1-diphenyl-2-trinitrophenylhydrazine (DPPH) free radical was determined. The IC50 values of essential oil of Artemisia annua in growth period and flower and fruit stage were 0. 40 and 1. 66 mg/m / L, respectively, for 0. 05 mmol/L DPPH solution. The results showed that the volatile oil of Artemisia annua during the growth period and flower and fruit period had good antioxidant activity.
【作者单位】：新能源转换与储存关键材料技术工信部重点实验室城市水资源与水环境国家重点实验室哈尔滨工业大学化工与化学学院;
【基金】：国家自然科学基金项目(Nos.21773050,21528501) 城市水资源与水环境国家重点实验室(哈尔滨工业大学)自主课题(No.2017DX05) 哈尔滨工业大学环境生态创新专项基金项目(No.HSCJ201607)资助~~
【分类号】：O629;O657.63

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