高精度自动化溶液制备系统的设计与实现

发布时间：2018-12-05 19:03

【摘要】：溶液制备是化学分析、医药卫生等实验室最基本的工作内容之一。虽然分析化学、生物分析等学科正在快速发展,但高精度的自动化溶液制备系统仍然发展缓慢,在精度要求较高的样品处理上大多还使用人工处理的方式。人工配液具有耗时长、操作繁琐、精度低等问题,因此,开发精度高且操作简便的自动化溶液制备系统取代人工配液必将成为一种趋势。本文在分析国内外溶液制备系统发展现状的基础上,设计了一种具有通用性的高精度自动化溶液制备系统,该系统具有精确配制、比例稀释、等量分配和可二次开发等功能,并且能根据需要将同一过程全自动地重复执行。溶液制备系统采用智能化设计,具有操作简单、配液方便、配液精度高等优点。系统由上位机模块、下位机通信模块、下位机主控模块、管道切换模块、管道切换模块驱动电路、自动进样器模块、自动进样器模块驱动电路7个部分组成。其中,下位机主控模块、管道切换模块、管道切换模块驱动电路、自动进样器模块和自动进样器模块驱动电路构成下位机执行系统,便于用户二次开发使用。下位机主控模块以STM32F103作为核心芯片,进行自动进样、管道切换的控制。下位机通信模块使用CC2540和STM32F103组合的控制板,实现无线接收手机指令并发送控制指令的功能。上位机模块采用Android手机,完成功能参数的设定与动作过程的显示。本文完成的主要工作包括以下几个方面:(1)查阅相关文献,分析当前溶液制备系统的原理与组成。以稀释过程为研究对象制定设计方案,选择了合适的柱塞泵、管道和切换阀。(2)针对精确配制、比例稀释、等量分配和二次开发功能,设计溶液制备系统硬件电路与软件程序,实现自动化高精度配液。(3)对溶液制备系统进行实验测试。通过比例稀释实验测试系统的取样精度、准确度与取样速度。通过等量分配实验测试系统稳定性。将实验结果分别进行原子吸收与质谱分析,分析结果表明,系统可以完全满足常规溶液配制的标准要求,不但取样准确、重复性好,而且也可以大量节省实验室人员的工作时间以及化学试剂的用量。
[Abstract]:Solution preparation is one of the most basic contents of chemical analysis, medicine and health laboratory. Although analytical chemistry, biological analysis and other disciplines are developing rapidly, high-precision automated solution preparation systems are still slow to develop, and manual processing is mostly used in sample processing with high precision. It is a trend to develop an automatic solution preparation system with high precision and simple operation to replace the artificial liquid dispensing system because of its long time consuming, complicated operation and low precision. Based on the analysis of the present situation of solution preparation system at home and abroad, a universal and high precision automatic solution preparation system is designed. The system has the functions of accurate preparation, proportional dilution, equal distribution and redevelopment. And can carry out the same process automatically and repeatedly according to the need. The solution preparation system is designed intelligently, which has the advantages of simple operation, convenient distribution and high precision. The system consists of upper computer module, lower computer communication module, master control module of lower computer, driving circuit of pipeline switching module and driving circuit of automatic sampler module. Among them, the main control module of the lower computer, the driving circuit of the pipeline switching module, the driving circuit of the automatic sampler module and the automatic sampler module constitute the execution system of the lower computer, which is convenient for the user to develop and use again. The main control module of the lower computer takes STM32F103 as the core chip to control automatic injection and pipeline switching. The communication module of the lower computer uses the control board combined with CC2540 and STM32F103 to realize the function of receiving and sending the command of the mobile phone wirelessly. The upper computer module uses Android mobile phone to complete the setting of function parameters and the display of action process. The main work of this paper includes the following aspects: (1) the principle and composition of the current solution preparation system are analyzed by consulting the relevant literature. Taking the dilution process as the object of study, the design scheme was formulated, and suitable piston pumps, pipes and switching valves were selected. (2) aiming at accurate preparation, proportional dilution, equal distribution and secondary development function, The hardware circuit and software program of the solution preparation system are designed to realize the automatic and high precision solution distribution. (3) the solution preparation system is tested experimentally. The sampling precision, accuracy and sampling speed of the system are measured by proportional dilution experiment. The stability of the system is tested by the equal distribution experiment. The experimental results were analyzed by atomic absorption spectrometry and mass spectrometry respectively. The results show that the system can completely meet the standard requirements of conventional solution preparation, and not only the sampling is accurate, but also the repeatability is good. And can also save a lot of laboratory staff working time and the amount of chemical reagents.
【学位授予单位】：东北师范大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：O645

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