UPLC结合化学计量法对不同产地黄芪的快速鉴别

发布时间：2018-12-09 16:00

【摘要】：目的建立UPLC-DAD法测定黄芪中4种黄酮类成分的测定方法,结合化学计量学方法快速鉴别黄芪的产地。方法采用UPLC法测定5个省份20批黄芪药材中毛蕊异黄酮苷、芒柄花苷、毛蕊异黄酮、芒柄花素4种黄酮类成分,运用聚类分析、主成分分析、判别分析方法综合分析不同产地黄芪质量。结果以毛蕊异黄酮苷、芒柄花苷、毛蕊异黄酮、芒柄花素4种黄酮类成分的量为标准,进行聚类分析与主成分分析,两者结果一致,可将不同黄芪产地区分为3大类。判别分析进一步以Fisher判别函数的形式将内蒙古、四川、吉林产地较准确进行区分,甘肃与陕西的样品出现了误判,初始分组的正确率为85%。结论 UPLC结合化学计量法可快速、准确鉴别黄芪产地,研究结果也可为不同产地黄芪的质量评价提供科学的依据。
[Abstract]:Objective to establish a UPLC-DAD method for the determination of four flavonoids in Astragalus membranaceus, and to identify the origin of Astragalus membranaceus by chemometrics. Methods four flavonoids in 20 batches of Astragalus membranaceus in five provinces were determined by UPLC. The four flavonoids were analyzed by cluster analysis and principal component analysis. The quality of Astragalus membranaceus from different areas was analyzed by discriminant analysis. Results the contents of four flavonoids, such as isoflavone glycoside, anthocyanin, isoflavone and anthocyanin, were taken as the standard. The results of cluster analysis and principal component analysis were consistent, and different Astragalus membranaceus could be divided into 3 groups. In the form of Fisher discriminant function, the samples from Inner Mongolia, Sichuan and Jilin were distinguished more accurately by discriminant analysis. The samples from Gansu and Shaanxi were misjudged, and the correct rate of initial grouping was 85%. Conclusion UPLC combined with chemometrics can quickly and accurately identify the origin of Astragalus membranaceus, and the results can provide scientific basis for the quality evaluation of Astragalus membranaceus from different areas.
【作者单位】：成都中医药大学药学院;中国中医科学院中药研究所;西华大学生物食品工程学院;
【基金】：国家自然科学基金项目(81202903,81573534) 国家中医药管理局中医药行业科研专项(201407003)
【分类号】：R284.1;O657.7

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