基于标准差传递规律的手机比色法测定亚甲基蓝溶液

发布时间：2018-12-17 16:59

【摘要】：采用基于误差传递规律的手机比色法测定亚甲基蓝溶液,根据颜色三原色值RGB与灰度值Gr之间的关系,通过标准差传递公式的运算,从理论上证实了间接测量法的可行性。分析了不同光照环境对采集照片质量的影响,将3款不同型号的手机作为采样工具进行对比。结果表明,间接测量法的精密度显著提高,3款手机图片颜色的间接测量值XR、XG、XB与亚甲基蓝质量浓度C之间的变化趋势一致,其中XB与质量浓度C成正比,据此建立了亚甲基蓝质量浓度测定的新方法。该方法对样品测定的相对标准偏差为2.7%~4.8%,加标回收率为91.3%~101%,其稳定性、精密度和准确度均符合测定需求。
[Abstract]:The methylene blue solution was determined by mobile phone colorimetry based on error transfer law. According to the relationship between the color trichromatic value RGB and gray value Gr, the feasibility of indirect measurement method was proved theoretically by the calculation of standard deviation transfer formula. The influence of different illumination environment on the quality of collecting photos was analyzed, and three different models of mobile phones were compared as sampling tools. The results showed that the precision of the indirect measurement method was significantly improved, and the change trend between the indirect measurement value XR,XG,XB and the mass concentration C of methylene blue was the same, and the XB was directly proportional to the mass concentration C. A new method for the determination of methylene blue was established. The relative standard deviation for the determination of samples by this method is 2.7 and 4.8, and the recovery rate is 91.3 and 101.The stability, precision and accuracy of the method are in line with the requirements of the determination.
【作者单位】：汉江师范学院生物化学与环境工程系;
【分类号】：O657.3

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