羌活与宽叶羌活药材的红外光谱鉴别
发布时间：2018-12-21 13:47
【摘要】：目的:建立红外光谱快速鉴别羌活和宽叶羌活两种基原植物药材的方法。方法:收集了8份羌活和4份宽叶羌活根茎和根药材,用傅里叶变换红外光谱技术测定4 000~400 cm~(-1)范围内的一维红外光谱和二维相关红外光谱,计算二阶导数光谱,定量测定峰的强度,并进行光谱解析、主成分分析。结果:羌活与宽叶羌活药材一维红外光谱的特征峰不同。羌活在1 739(吸光度为0.39)、1 428(0.46)、1 076(0.91)、863(0.03)、764(0.08)cm~(-1)处有明显吸收峰,而宽叶羌活在此处的峰不明显(强度小于阈值0.01);宽叶羌活在1 719(0.34)、1 607(0.50)、1 444(0.41)、823(0.06)、775(0.07)cm~(-1)处的吸收峰明显,而羌活在此处的峰不明显。在模式识别-主成分分析聚类图中,羌活与宽叶羌活分布于不同区域。二阶导数谱中两者主要共有峰的强度明显不同,羌活在1 747、1 468、1 159、1 078和988 cm~(-1)的峰强度明显高于宽叶羌活。而宽叶羌活在1 627、1 605、1 568、1 512和1 269 cm~(-1)峰强度明显高于羌活。二维相关红外光谱中两者自动峰的数量、位置及其相互关系不同。羌活样品在850~1 500 cm~(-1)范围内有13个自动峰,而宽叶羌活为9个自动峰。结论:根据一维、二阶导数和二维相关红外光谱,羌活与宽叶羌活两种植物的药材可快速鉴别。峰强度定量比较、主成分分析、聚类分析增加了鉴别的客观性和准确性。
[Abstract]:Objective: to establish a method for rapid identification of Radix Notopterygii and Radix Notopterygii. Methods: eight samples of Radix Notopterygium and four samples of Radix Notopterygii rhizome and root were collected. The 1D and 2D IR spectra in the range of 400 cm~ (-1) were determined by Fourier transform infrared spectroscopy (FTIR), and the second derivative spectra were calculated. The intensity of the peak was determined quantitatively, and the spectral analysis and principal component analysis were carried out. Results: the characteristic peaks of one-dimensional infrared spectrum of Radix Notopterygii and Radix Notopterygii were different. There are obvious absorption peaks at 1 739 (absorbance 0. 39), 1 428 (0. 46), 1 076 (0. 91), 863 (0. 03), 764 (0. 08) cm~ (-1). But the peak of Notopterygium gibbergii was not obvious here (the intensity was less than the threshold value 0.01). The absorption peaks at 1 719 (0. 34), 1 607 (0. 50), 1 444 (0. 41), 823 (0. 06), 775 (0. 07) cm~ (-1) were obvious, but the peaks of Notopterygium Notopterygii were not obvious. In the pattern recognition-principal component analysis (PCA) cluster map, Notopterygium gibbergii and Notopterygium gibbergii are distributed in different regions. The intensity of the main common peaks in the second derivative spectrum was obviously different. The peak intensity of Notopterygium chinensis at 1 468 9 cm~ (-1 078) and 988 cm~ (-1) was significantly higher than that of Notopterygium gibbergii. However, the peak intensities of 1 627, 1 605 and 1 269 cm~ (-1) peaks of Notopterygium gibbergii were significantly higher than those of Notopterygium gibbergii. The number, position and relationship of the automatic peaks in two dimensional correlation infrared spectroscopy are different. In the range of 850 ~ 1 500 cm~ (-1), there are 13 automatic peaks in the sample of Notopterygium, and 9 peaks in the range of 1 500 cm~ (-1). Conclusion: according to one dimensional, second derivative and two dimensional correlation infrared spectroscopy, two kinds of medicinal herbs of Notopterygium and Notopterygium chinensis can be quickly identified. Quantitative comparison of peak intensity, principal component analysis and cluster analysis increased the objectivity and accuracy of identification.
【作者单位】： 成都中医药大学药学院/中药材标准化教育部重点实验室;四川省食品药品检验检测院;四川省中医药科学院;珀金埃尔默企业管理(上海)有限公司;
【基金】：国家基础科学人才培养基金项目(J1310034-02) 四川省科技支撑计划项目(2014SZ0071-4) 成都中医药大学大学生科研实践创新课题(ky2016-016)
【分类号】：O657.33;R284.1
，
本文编号：2388992
[Abstract]:Objective: to establish a method for rapid identification of Radix Notopterygii and Radix Notopterygii. Methods: eight samples of Radix Notopterygium and four samples of Radix Notopterygii rhizome and root were collected. The 1D and 2D IR spectra in the range of 400 cm~ (-1) were determined by Fourier transform infrared spectroscopy (FTIR), and the second derivative spectra were calculated. The intensity of the peak was determined quantitatively, and the spectral analysis and principal component analysis were carried out. Results: the characteristic peaks of one-dimensional infrared spectrum of Radix Notopterygii and Radix Notopterygii were different. There are obvious absorption peaks at 1 739 (absorbance 0. 39), 1 428 (0. 46), 1 076 (0. 91), 863 (0. 03), 764 (0. 08) cm~ (-1). But the peak of Notopterygium gibbergii was not obvious here (the intensity was less than the threshold value 0.01). The absorption peaks at 1 719 (0. 34), 1 607 (0. 50), 1 444 (0. 41), 823 (0. 06), 775 (0. 07) cm~ (-1) were obvious, but the peaks of Notopterygium Notopterygii were not obvious. In the pattern recognition-principal component analysis (PCA) cluster map, Notopterygium gibbergii and Notopterygium gibbergii are distributed in different regions. The intensity of the main common peaks in the second derivative spectrum was obviously different. The peak intensity of Notopterygium chinensis at 1 468 9 cm~ (-1 078) and 988 cm~ (-1) was significantly higher than that of Notopterygium gibbergii. However, the peak intensities of 1 627, 1 605 and 1 269 cm~ (-1) peaks of Notopterygium gibbergii were significantly higher than those of Notopterygium gibbergii. The number, position and relationship of the automatic peaks in two dimensional correlation infrared spectroscopy are different. In the range of 850 ~ 1 500 cm~ (-1), there are 13 automatic peaks in the sample of Notopterygium, and 9 peaks in the range of 1 500 cm~ (-1). Conclusion: according to one dimensional, second derivative and two dimensional correlation infrared spectroscopy, two kinds of medicinal herbs of Notopterygium and Notopterygium chinensis can be quickly identified. Quantitative comparison of peak intensity, principal component analysis and cluster analysis increased the objectivity and accuracy of identification.
【作者单位】： 成都中医药大学药学院/中药材标准化教育部重点实验室;四川省食品药品检验检测院;四川省中医药科学院;珀金埃尔默企业管理(上海)有限公司;
【基金】：国家基础科学人才培养基金项目(J1310034-02) 四川省科技支撑计划项目(2014SZ0071-4) 成都中医药大学大学生科研实践创新课题(ky2016-016)
【分类号】：O657.33;R284.1
，
本文编号：2388992
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