黑穗醋栗果实多糖的制备、结构鉴定及生物活性研究

发布时间：2018-12-23 13:20

【摘要】：以黑穗醋栗果实为原料,采用超声波法提取黑穗醋栗果实粗多糖。利用大孔树脂D4006,阴离子交换剂Q-Sepharose FF和葡聚糖凝胶Sephadex G-100对粗多糖进行分离纯化,得到均一黑穗醋栗多糖(black currant polysaccharide,BCP)。高效液相色谱法测定BCP的重均分子质量为16 329 k D。气相色谱法测定BCP单糖组成及物质的量比为:n(半乳糖醛酸)∶n(鼠李糖)∶n(阿拉伯糖)∶n(木糖)∶n(甘露糖)∶n(葡萄糖)∶n(半乳糖)=0.31∶0.66∶1.63∶0.36∶0.23∶0.31∶1.00。傅里叶变换红外光谱分析表明BCP含有多糖的特征吸收峰,紫外光谱和定性实验表明BCP不含花色苷、蛋白质、核酸和淀粉。刚果红实验表明BCP不具有三螺旋结构。生物活性研究结果表明:在0.2~1.2 mg/m L范围内,BCP对1,1-二苯基-2-三硝基苯肼自由基、·OH、超氧阴离子自由基(O2-·)均有较明显的清除作用,随着质量浓度增大,清除率明显升高。BCP对糖基化反应3个阶段产物(Amadori产物、二羰基化合物和糖基化终产物)的形成均表现出良好的抑制作用,抑制率高于阳性对照氨基胍,且随质量浓度的增加,抑制率升高。BCP对α-淀粉酶具有一定的抑制活性,但抑制效果低于阳性对照阿卡波糖。
[Abstract]:The crude polysaccharides from black currant fruit were extracted by ultrasonic method. The crude polysaccharides were separated and purified by macroporous resin D4006, anion exchange agent Q-Sepharose FF and dextran gel Sephadex G-100, and (black currant polysaccharide,BCP was obtained. The weight average molecular weight of BCP was 16 329 KD by HPLC. Gas chromatographic determination of BCP monosaccharide composition and molar ratio of: n (galacturonic acid,): n (rhamnose,): n (arabinose,): n (xylose,): n (mannose,): n (glucose,): n (galactose) = 0.31: 0.66: 1.63: 0.36: 0.23: 0.31: 1.00. Fourier transform infrared spectroscopy (FTIR) showed that BCP contained the characteristic absorption peak of polysaccharides. UV spectra and qualitative experiments showed that BCP did not contain anthocyanins, proteins, nucleic acids and starch. Congo red experiment shows that BCP does not have trihelix structure. The results showed that in the range of 0.2 mg/m / L, BCP had obvious scavenging effects on 1-diphenyl-2-trinitrophenylhydrazine radical and OH, superoxide anion radical (O2-). With the increase of mass concentration, the clearance rate increased obviously. BCP showed a good inhibitory effect on the formation of Amadori products, dicarbonyl compounds and glycosylation end products, and the inhibition rate was higher than that of positive control, aminoguanidine. The inhibition rate increased with the increase of mass concentration. BCP had a certain inhibitory activity on 伪 -amylase, but the inhibitory effect was lower than that of the positive control.
【作者单位】：东北农业大学理学院;
【基金】：国家自然科学基金青年科学基金项目(31600276) 黑龙江省自然科学基金面上项目(C2015004) 黑龙江省SITP计划项目(201610224112)
【分类号】：O629.12

【相似文献】