牛血清蛋白对二硬脂酰基磷脂酰乙醇胺单层膜结构的影响

发布时间：2018-12-24 07:32

【摘要】：利用Langmuir-Blodgett(LB)技术结合原子力显微镜(AFM),研究了牛血清蛋白(BSA)在气/液界面上对二硬脂酰基磷脂酰乙醇胺(DSPE)单层膜结构的影响.通过改变亚相的pH值和BSA浓度,获得了不同条件下DSPE单层膜的等温线、吸附曲线和压缩循环曲线等.实验结果表明,亚相中BSA的存在对DSPE单层膜的压缩性、稳定性以及相变行为产生了较大的影响.吸附动力学结果表明,DSPE单层膜对BSA分子的吸附量存在一定的阈值,且该阈值的大小与亚相pH值相关.通过分析实验数据可知,当亚相pH=3时,BSA的疏水残基几乎全部暴露在外面,2种分子之间的相互作用最强;而pH=7时,BSA仅有少量的疏水残基暴露在外面,2种分子之间的相互作用最弱.原子力显微镜观测到的单层膜形态变化特点与曲线分析结果一致.该研究为了解牛血清蛋白与磷脂分子之间的相互作用机理提供了重要的实验基础和理论依据.
[Abstract]:The effect of bovine serum protein (BSA) on the structure of (DSPE) monolayer membrane was studied by Langmuir-Blodgett (LB) technique and atomic force microscopy (AFM). The isotherms, adsorption curves and compression cycle curves of DSPE monolayer films under different conditions were obtained by changing the pH value and BSA concentration of the subphase. The experimental results show that the existence of BSA in subphase has a great influence on the compressibility, stability and phase transition behavior of DSPE monolayer films. The adsorption kinetics results show that there is a certain threshold for the adsorption of BSA molecules by DSPE monolayer membrane, and the magnitude of the threshold is related to the pH value of the subphase. By analyzing the experimental data, the hydrophobic residues of BSA were almost exposed to the outside when the subphase pH= _ 3 was 3, and the interaction between the two molecules was the strongest. In pH= 7, only a small amount of hydrophobic residues of BSA were exposed to the outside, and the interaction between the two molecules was the weakest. The morphology of monolayer films observed by atomic force microscope is consistent with the curve analysis. This study provides an important experimental and theoretical basis for understanding the interaction mechanism between bovine serum protein and phospholipid molecules.
【作者单位】：陕西师范大学物理学与信息技术学院;
【基金】：国家自然科学基金(批准号:21402114) 陕西省自然科学基础研究计划项目(批准号:2016JM2010) 中央高校基本科研业务费专项(批准号:2017CSY004,GK201603026) 国家大学生创新创业训练计划项目(批准号:CX17189)资助~~
【分类号】：O641.3

【相似文献】