毛细管区带电泳化学发光法测定食品中残留的磺胺类药物

发布时间：2019-01-05 07:48

【摘要】：基于碱性介质中,磺胺类药物对Ag?配合物与鲁米诺(Luminol,Lu)化学发光体系的发光强度有抑制作用,建立了毛细管电泳-化学发光分离检测磺胺甲VA唑(Sulfamethoxazole,SMZ)、磺胺二甲氧嘧啶(Sulfadimethoxine,SDM)、磺胺噻唑(Sulfathiazole,ST)的方法。3种磺胺类药物经毛细管电泳分离后,分别与Ag?配合物和鲁米诺化学发光体系作用,以相对迁移时间定性,相对化学发光强度定量,采用标准曲线法测定样品中的待测物含量。对影响毛细管电泳的分离与化学发光检测的条件均进行了优化。在最佳分离检测条件下,3种磺胺类药物在2.0~200μg/m L范围内线性良好(r0.9977)。对3种磺胺类药物平行测定7次,相对标准偏差(RSD)为1.3%~1.9%,3种磺胺类物质的检出限分别为0.33,0.20和0.034μg/m L,加标回收率为80.2%~102.9%。将本方法应用于猪肉、鸡肉、牛奶中的3种磺胺类药物残留量检测,结果令人满意。
[Abstract]:Application of Sulfonamides to Ag? in Alkaline Media The luminescence intensity of the complex with the luminol (Luminol,Lu) chemiluminescence system was inhibited. A capillary electrophoresis chemiluminescence separation method was established for the detection of sulfamethoxazole (Sulfamethoxazole,SMZ), sulfamethoxypyrimidine (Sulfadimethoxine,SDM) and sulfamethoxazole (Sulfathiazole,). Three sulfonamides were separated from Ag? by capillary electrophoresis. The interaction of the complexes with the luminol chemiluminescence system was characterized by relative transfer time and quantitative analysis of relative chemiluminescence intensity. The content of the sample was determined by standard curve method. The conditions affecting the separation and chemiluminescence detection of capillary electrophoresis were optimized. Under the optimum conditions of separation and detection, the linearity of the three sulfonamides was good in the range of 2.0 渭 g / mL (r 0.9977). The relative standard deviation (RSD) of the three sulfonamides was 1. 3 and 1. 9, respectively. The detection limits of the three sulfonamides were 0. 33 渭 g / mL and 0.034 渭 g / mL, respectively. The recoveries of the three sulfonamides were 80.2% and 102.9%, respectively. The method has been applied to the determination of sulfa residues in pork, chicken and milk with satisfactory results.
【作者单位】：河北医科大学公共卫生学院;
【基金】：国家自然科学基金项目(Nos.81273128,81402723)资助~~
【分类号】：TS207.5;O657.3

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