MOF材料负载酶及其催化性能研究

发布时间：2019-01-26 20:50

【摘要】：金属-有机骨架材料是一种近二十年来新兴的纳米多孔材料,结构类似于沸石,具有较高的孔隙率与比表面积,已在能源气体存储、吸附分离、膜分离、多相催化、药物控释和光电磁等领域广泛应用。此外,将MOF材料用于酶固定,可以解决酶难以回收、无法重复使用等不足。酶固定指的是采用载体负载酶,例如吸附、包埋等方法,使酶限制于载体的表面以及微孔中,同时负载后的酶仍具有与游离酶类似的催化活性,以达到回收酶重复利用的目的。酶固定具有很多优点,如,固定化酶具有更好的稳定性,易于储存和运输,并且可以反复使用,实现重复利用。本工作通过实验的方法,采用了模板剂法溶剂热合成多级孔UiO-66,与文献发表的材料一致,BET为917 m2.g-1。进一步用其负载了胃蛋白酶。负载方法为吸附法,在35℃,pH=3.6的负载条件下,多级孔UiO-66、PCN-222、SBA-15固定胃蛋白酶的最大吸附量分别为0.96 g/g、0.755 g/g、0.366 g/g.本工作以血红蛋白作为底物,测定了胃蛋白酶和固定化酶的酶活,并且考察了固定化酶的重复利用性。测得浓度分别为1 mg/mL和0.8 mg/mL时的胃蛋白酶游离酶活分别为17.0×103U/g和18.5×103U/g.多级孔UiO-66吸附固定2 mg/mL胃蛋白酶的酶活为：1.1×103U/g.PCN-222和SBA-15固定后酶活较低。35℃下,在pH为3.6的醋酸缓冲液环境下进行酶催化反应,1h后,过滤出固体,用醋酸缓冲液洗净冻干。用上述方法测得酶活为0.65×103U/g,酶活回收率为56.5%.同样条件下反应2h,过滤洗净冻干后,测得酶活为0.57×103U/g,酶活回收率为49.3%.
[Abstract]:The metal-organic skeleton material is a new kind of nano-porous material in recent 20 years. Its structure is similar to that of zeolite. It has high porosity and specific surface area. It has been used in energy gas storage, adsorption and separation, membrane separation, and heterogeneous catalysis. Drug controlled release and optoelectronic magnetism are widely used. In addition, the application of MOF material to enzyme fixation can solve the problems of enzyme recovery and reuse. Enzyme immobilization refers to the use of carrier loading enzymes, such as adsorption, entrapping, etc., so that the enzyme is confined to the surface and micropore of the carrier, while the enzyme after loading still has the same catalytic activity as the free enzyme, in order to achieve the purpose of reusing the enzyme. Enzyme fixation has many advantages, such as better stability, easy storage and transport, and can be reused. In this work, the method of templating was used to synthesize multiporous UiO-66, by solvothermal method, and the BET was 917m2.g-1m ~ (-1). It is further loaded with pepsin. The maximum adsorption capacity of pepsin immobilized by multipore UiO-66,PCN-222,SBA-15 was 0.96 g / g 0.755 g / g 0.366 g / g at 35 鈩，

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