两基原中药决明子UPLC指纹图谱研究
发布时间：2019-02-09 14:29
【摘要】：目的建立两基原(决明、小决明)中药决明子的UPLC指纹图谱,为决明子药材质量控制和评价提供依据。方法采用Agilent ZORBAX EP C18(100 mm×3.0 mm,1.8μm)色谱柱;以乙腈-0.01%甲酸水溶液为流动相,梯度洗脱,体积流量0.4 m L/min,检测波长285 nm,柱温30℃,进样量为1μL。结果首次建立了决明子UPLC指纹图谱共有模式,运用相似度评价软件标定了20个共有峰,结合保留时间和紫外光谱分析,指认了7个峰;20批决明子药材相似度均大于0.9;聚类分析结果为当欧式距离平方和为5时,20批决明子样品可分为4类;主成分分析降维得到3个主成分,根据主成分得分将样品进行划分归类,与聚类分析结果一致;通过拟合归纳第一主成分的载荷因子模型,筛选出红链霉素-6-O-β-龙胆二糖苷等具有评价决明子质量优劣的特征峰。结论决明与小决明共有化学成分存在共性差异,该方法可以用于决明子药材质量控制和评价。
[Abstract]:Objective to establish the UPLC fingerprint of Cassia sinensis, and to provide basis for quality control and evaluation of Cassia sinensis. Methods Agilent ZORBAX EP C18 (100 mm 脳 3.0 mm,1.8 渭 m) column was used, acetonitrile-0.01% formic acid solution was used as mobile phase, gradient elution, volume flow rate 0.4 mL / min, detection wavelength 285 nm, column temperature 30 鈩，
本文编号：2419052
[Abstract]:Objective to establish the UPLC fingerprint of Cassia sinensis, and to provide basis for quality control and evaluation of Cassia sinensis. Methods Agilent ZORBAX EP C18 (100 mm 脳 3.0 mm,1.8 渭 m) column was used, acetonitrile-0.01% formic acid solution was used as mobile phase, gradient elution, volume flow rate 0.4 mL / min, detection wavelength 285 nm, column temperature 30 鈩，
本文编号：2419052
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