益心舒片的HPLC指纹图谱
发布时间：2019-02-13 01:20
【摘要】：目的:建立益心舒片的HPLC指纹图谱,为益心舒片的质量评价提供方法。方法:样品经75%甲醇提取后,采用Waters Symmetry ShieildTMC18色谱柱(4.6 mm×250 mm,5μm)进行检测,以乙腈-0.5%甲酸水为流动相,梯度洗脱,流速1.0 m L·min~(-1),检测波长260 nm,柱温30℃。采用国家药典委员会出版的"中药色谱指纹图谱相似度评价系统"(2012年版),对10批不同批次的益心舒片化学成分指纹图谱进行相似度计算,并且通过对照品比对及高效液相与质谱联用技术(HPLC-MS)对主要共有峰进行指认。结果:10批益心舒片指纹图谱中有29个共有峰,各峰分离度良好,各批次间共有峰的相对保留时间RSD均1.0%,样品间相似度均0.9,共指认出12个峰。结论:该方法具有良好的精密度、重复性、稳定性,各共有峰间分离度高,可用于益心舒片的质量综合评价。
[Abstract]:Objective: to establish the HPLC fingerprint of Yixinshu tablets and to provide a method for the quality evaluation of Yixinshu tablets. Methods: the samples were extracted with 75% methanol and detected on a Waters Symmetry ShieildTMC18 column (4.6 mm 脳 250 mm,5 渭 m). Acetonitrile-0.5% formic acid water was used as mobile phase, gradient elution was used, flow rate was 1.0 mL min~ (-1). The detection wavelength was 260 nm, and the column temperature was 30 鈩，
本文编号：2421021
[Abstract]:Objective: to establish the HPLC fingerprint of Yixinshu tablets and to provide a method for the quality evaluation of Yixinshu tablets. Methods: the samples were extracted with 75% methanol and detected on a Waters Symmetry ShieildTMC18 column (4.6 mm 脳 250 mm,5 渭 m). Acetonitrile-0.5% formic acid water was used as mobile phase, gradient elution was used, flow rate was 1.0 mL min~ (-1). The detection wavelength was 260 nm, and the column temperature was 30 鈩，
本文编号：2421021
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