上转换荧光猝灭试纸条检测牛奶中磺胺喹恶啉

发布时间：2019-02-23 10:27

【摘要】：目的:根据荧光共振能量转移(fluorescence resonance energy transfer,FRET)的原理,构建一种基于上转换荧光纳米材料的新型免疫标记荧光猝灭试纸条检测牛奶中的兽药磺胺喹恶啉(sulfaquinoxaline,SQX)残留。方法:通过热分解方法合成上转换纳米荧光材料,通过柠檬酸三钠还原法制备胶体金纳米颗粒。将合成的上转换荧光颗粒固定在试纸条C线上,并将上转换荧光颗粒与SQX包被抗原的混合物固定在T线上,将胶体金标记的SQX单克隆抗体喷涂在金标垫上。滴加样品后,随着样品液的流动,金标抗体会移动到T线处并与SQX包被抗原结合,这种结合会导致抗体上的纳米金颗粒(受体)与T线上的上转换颗粒靠近(供体),从而发生FRET,产生荧光猝灭。在980 nm激发器激发波长下通过目测检测荧光强弱变化,从而实现对SQX的检测。结果:该免疫荧光猝灭试纸条检测SQX的方法检测限为1μg/L,牛奶样品的检测限为8μg/L。整个检测过程不超过15 min。结论:该方法操作简便、灵敏度高、检测时间短、结果易于判断,可以满足牛奶中SQX残留的现场快速检测的要求。
[Abstract]:Aim: based on the principle of fluorescence resonance energy transfer (fluorescence resonance energy transfer,FRET), a novel immunofluorescence quenching strip based on up-conversion fluorescent nanomaterials was constructed for the detection of sulfamethoxaline (sulfaquinoxaline,SQX) residues in milk. Methods: colloidal gold nanoparticles were synthesized by thermal decomposition method and colloidal gold nanoparticles were prepared by trisodium citrate reduction method. The synthesized up-conversion fluorescent particles were immobilized on the C line of the test strip and the mixture of the up-conversion fluorescent particles and the SQX coated antigen was fixed on the T line. The colloidal gold-labeled SQX monoclonal antibody was sprayed on the gold label pad. After dropping the sample, as the sample fluid flows, the gold-labeled antibody moves to the T line and binds to the SQX coated antigen, which causes the nanoparticles (receptor) on the antibody to be close to the up-conversion particle on the T line (donor). Thus, FRET, produced fluorescence quenching. Under the excitation wavelength of 980 nm exciter, the change of fluorescence intensity is detected by visual measurement, thus the detection of SQX is realized. Results: the detection limit of SQX by immunofluorescence quenching strip was 1 渭 g / L, and that of milk samples was 8 渭 g / L. The whole detection process does not exceed 15 min. Conclusion: this method is easy to operate, has high sensitivity, short detection time and is easy to judge. It can meet the requirements of rapid detection of SQX residues in milk.
【作者单位】：天津科技大学食品工程与生物技术学院教育部食品营养与安全重点实验室;
【基金】：国家国际科技合作专项(2014DFR30350) 天津市科技计划项目(14ZCDGNC00098)
【分类号】：O657.3;TS252.7

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