羟基磷灰石晶体生长的调控研究
发布时间：2019-03-12 17:49
【摘要】：羟基磷灰石(简称HAP或HA),其组成和结构与颈椎动物的骨骼和牙齿基本一致。同时,HA具有良好的生物相容性、生物活性、骨传导性、骨增殖性、无毒性,常被用于骨组织的替代材料和生物体内载体材料。在其他领域中,由于HA具有良好的离子交换性和吸附性,常用于处理生活用水中含量过高的氟离子,或者处理一些废水中的重金属离子。HA陶瓷的耐热、耐湿范围比较宽,抗衰老性能比较好,可作为湿敏半导体器件。这些性能是由HA的形貌、结构和组成决定,因此,科学工作者们试图制备出特定形貌的HA。本文中应用了自组装技术引入功能基团,例如引入氨基、羟基、羧基自组装分子膜(SAMs)。SAMs接枝至钛基底上,获得平滑的功能界面。在功能界面上成功地水热合成出均匀且无裂缝的HA涂层。通过AFM和XPS对界面进行分析;用XRD、SEM、TEM对HA进行物相和形貌等进行分析。结果表明,此方法成功地在接枝了氨基、羟基、羧基自组装分子膜的钛基底上制备出高晶度、紧密的、定向的HA涂层。接枝了羧基的自组装分子膜的钛基底比氨基、羟基的更用利于羟基磷灰石的成核。探讨了不同的反应时间、pH、反应温度对HA涂层的成核和生长的调控。这种方法成功的合成出高晶度、紧密的、附着的HA涂层。此外,体外细胞评价实验表明,和未处理钛基底对比,HA涂层增强细胞的黏附性。研究了在高的pH值情况下,乙二胺四乙酸二钠(Na_2EDTA)对HA晶体生长的影响,反应原料为无水氯化钙,磷酸氢二钠,按照不同浓度加入乙二胺四乙酸二钠,调节溶液的pH,探讨HA形貌和结构变化。研究表明,高的pH能够促进HA微球形成,降低pH值,微球的紧密度逐渐下降;当Na_2EDTA/Ca为2:2时候,有利于紧密的微球结构形成;同时反应温度也影响着微球的紧密程度。探讨了在高的pH,Na_2EDTA存在下,HA微球的形成机理。采用一种新的方法,氨水扩散法,合成多孔球形HA。用XRD、SEM、TEM、FT-IR和BET对HA进行形貌、结构和物相等分析。结果表明,氨水扩散调节反应液的pH,促进HA晶体的形成;反应时间以及温度对HA晶体的形貌起到很好的控制。在60℃氨水扩散24 h条件下,成功地制备出多孔的大比表面积HA微球。利用不同反应时间,提出了多孔HA微球的形成机理。同时,探讨了多孔HA微球作为吸附剂,对重金属铅离子和铜离子的吸附性能,对Pb~(2+)和Cu~(2+)的吸附量分别为213.58±2.63和59.68±3.44 mg/g。根据上面的氨水扩散法,构思了一种新的思路来合成多孔中空的HA微球。研究表明Na_2EDTA能够控制Ca~(2+)离子在合成溶液中释放和分布,促进中空结构的形成;同时反应时间也在一定程度上影响着中空结构形成。根据不同时间,对多孔中空HA微球形成机理进行分析。
[Abstract]:The composition and structure of hydroxyapatite (HAP or HA),) are basically consistent with the bones and teeth of cervical vertebrae. At the same time, HA has good biocompatibility, bioactivity, bone conductivity, bone proliferation, non-toxicity, often used in bone tissue substitute materials and biological carrier materials. In other fields, because of its good ion exchange and adsorption properties, HA is often used to treat excessive fluoride ions in domestic water, or to treat heavy metal ions in some wastewater. Ha ceramics have a wide range of heat resistance and humidity resistance. Anti-aging performance is better, can be used as humidity-sensitive semiconductor devices. These properties are determined by the morphology, structure and composition of HA. Therefore, scientists try to produce HA. with specific morphology. In this paper, the self-assembly technique was used to introduce functional groups, such as amino, hydroxyl and carboxyl self-assembled molecular membrane (SAMs). SAMs was grafted onto titanium substrate to obtain a smooth functional interface. The uniform and crack-free HA coating was successfully synthesized on the functional interface by hydrothermal synthesis. The interface was analyzed by AFM and XPS, and the phase and morphology of HA were analyzed by XRD,SEM,TEM. The results showed that the HA coatings with high crystallinity, compact orientation and orientation were successfully prepared on titanium substrates grafted with amino, hydroxyl and carboxyl self-assembled molecular films. The titanium substrate of the self-assembled molecular membrane grafted with carboxyl group is more favorable to the nucleation of hydroxyapatite than the amino group and the hydroxyl group is more favorable to the nucleation of hydroxyapatite. The effects of different reaction time and pH, reaction temperature on the nucleation and growth of HA coating were discussed. This method successfully synthesized high crystallinity, compact, attached HA coating. In addition, cell evaluation in vitro showed that the HA coating enhanced the adhesion of the cells compared with the untreated titanium substrate. The effect of disodium ethylenediaminetetraacetate (Na_2EDTA) on the growth of HA crystal was studied at high pH value. The raw materials were anhydrous calcium chloride and disodium hydrogen phosphate, adding disodium ethylenediamine tetraacetate according to different concentration to adjust the pH, of the solution. The morphology and structure of HA were investigated. The results show that high pH can promote the formation of HA microspheres, decrease the pH value and decrease the density of the microspheres, and when the Na_2EDTA/Ca is 2: 2, it is beneficial to the formation of tight microspheres structure. At the same time, the reaction temperature also affects the degree of compactness of the microspheres. The formation mechanism of HA microspheres in the presence of high pH,Na_2EDTA was discussed. Porous spherical HA. was synthesized by ammonia diffusion method with a new method. The morphology, structure and content of HA were analyzed by XRD,SEM,TEM,FT-IR and BET. The results show that the pH, of the reaction solution can promote the formation of HA crystal, and the reaction time and temperature can control the morphology of HA crystal. Porous HA microspheres with large specific surface area were successfully prepared under the condition of ammonia diffusion at 60 鈩，
本文编号：2439016
[Abstract]:The composition and structure of hydroxyapatite (HAP or HA),) are basically consistent with the bones and teeth of cervical vertebrae. At the same time, HA has good biocompatibility, bioactivity, bone conductivity, bone proliferation, non-toxicity, often used in bone tissue substitute materials and biological carrier materials. In other fields, because of its good ion exchange and adsorption properties, HA is often used to treat excessive fluoride ions in domestic water, or to treat heavy metal ions in some wastewater. Ha ceramics have a wide range of heat resistance and humidity resistance. Anti-aging performance is better, can be used as humidity-sensitive semiconductor devices. These properties are determined by the morphology, structure and composition of HA. Therefore, scientists try to produce HA. with specific morphology. In this paper, the self-assembly technique was used to introduce functional groups, such as amino, hydroxyl and carboxyl self-assembled molecular membrane (SAMs). SAMs was grafted onto titanium substrate to obtain a smooth functional interface. The uniform and crack-free HA coating was successfully synthesized on the functional interface by hydrothermal synthesis. The interface was analyzed by AFM and XPS, and the phase and morphology of HA were analyzed by XRD,SEM,TEM. The results showed that the HA coatings with high crystallinity, compact orientation and orientation were successfully prepared on titanium substrates grafted with amino, hydroxyl and carboxyl self-assembled molecular films. The titanium substrate of the self-assembled molecular membrane grafted with carboxyl group is more favorable to the nucleation of hydroxyapatite than the amino group and the hydroxyl group is more favorable to the nucleation of hydroxyapatite. The effects of different reaction time and pH, reaction temperature on the nucleation and growth of HA coating were discussed. This method successfully synthesized high crystallinity, compact, attached HA coating. In addition, cell evaluation in vitro showed that the HA coating enhanced the adhesion of the cells compared with the untreated titanium substrate. The effect of disodium ethylenediaminetetraacetate (Na_2EDTA) on the growth of HA crystal was studied at high pH value. The raw materials were anhydrous calcium chloride and disodium hydrogen phosphate, adding disodium ethylenediamine tetraacetate according to different concentration to adjust the pH, of the solution. The morphology and structure of HA were investigated. The results show that high pH can promote the formation of HA microspheres, decrease the pH value and decrease the density of the microspheres, and when the Na_2EDTA/Ca is 2: 2, it is beneficial to the formation of tight microspheres structure. At the same time, the reaction temperature also affects the degree of compactness of the microspheres. The formation mechanism of HA microspheres in the presence of high pH,Na_2EDTA was discussed. Porous spherical HA. was synthesized by ammonia diffusion method with a new method. The morphology, structure and content of HA were analyzed by XRD,SEM,TEM,FT-IR and BET. The results show that the pH, of the reaction solution can promote the formation of HA crystal, and the reaction time and temperature can control the morphology of HA crystal. Porous HA microspheres with large specific surface area were successfully prepared under the condition of ammonia diffusion at 60 鈩，
本文编号：2439016
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