肉制品中6种人工合成色素的快速测定

发布时间：2019-03-27 15:43

【摘要】：目的:建立一种同时测定肉制品中柠檬黄、苋菜红、胭脂红、日落黄、诱惑红和酸性红6种人工合成色素的高效液相色谱法。方法:采用乙醇-氨溶液提取样品中的色素,经乙醚脱脂,用Agilent Poroshell 120 SB-C_(18)色谱柱分离,以乙腈-乙酸铵(0.02 mol/L)为流动相,二极管阵列检测器可变波长程序测定。结果:6种色素在浓度0~10μg/mL均有良好的线性关系,线性系数均大于0.9999,加标回收率为70.33%~93.08%,检出限为0.12~0.21 mg/kg,相对标准偏差均小于5%。结论:该方法操作简单、快捷,重现性好,灵敏度高,可同时测定肉制品中6种人工合成色素,适用于肉制品的快速检测。
[Abstract]:Objective: To establish a high performance liquid chromatography (HPLC) for the simultaneous determination of 6 kinds of synthetic pigments in the meat products such as lemon yellow, vegetable red, carmine, sunset yellow, seduced red and acid red. Methods: The pigment in the sample was extracted with ethanol-ammonia solution, and the pigment was defatted with ether and separated by Agilent Portoshell 120 SB-C _ (18) column, and the acetonitrile-ethylene acid solution (0.02 mol/ L) was used as the mobile phase, and the diode array detector was determined by the variable wavelength program. The results showed that 6 kinds of pigments had a good linear relationship at the concentration of 0-10 & mu; g/ mL, the linear coefficient was more than 0.9999, the recovery rate of the spike was 70.33%-93.08%, the detection limit was 0.12-0.21 mg/ kg, and the relative standard deviation was less than 5%. Conclusion: The method is simple, rapid, good in reproducibility and high in sensitivity. Six synthetic pigments in meat products can be measured at the same time, and the method is suitable for rapid detection of meat products.
【作者单位】：贵港出入境检验检疫局;广西出入境检验检疫局检验检疫技术中心;
【基金】：基金项目:广西畜牧产品中非法添加色素风险评估及检测方法研究与应用(桂科重14121003-4-3)
【分类号】：O657.72;TS251.7

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