HPLC法快速测定8种中药中齐墩果酸和熊果酸

发布时间：2019-04-04 14:02

【摘要】：目的建立一种快速分析方法,同时测定中药材马鞭草Verbena officinalis、女贞子Ligustrum lucidum、夏枯草Prunella vulgaris、白花蛇舌草Hedyotis diffusa、败酱草Patrinia scabiosaefolia、枇杷叶Eriobotrya japonica、山楂Crataegus pinnatifida、木瓜Chaenomeles Fructus papaya中齐墩果酸和熊果酸的量。方法使用快速溶剂萃取法,采用甲醇作为提取溶剂,静态萃取时间6 min,制备样品溶液,液相色谱分析采用Acclaim C_(30)色谱柱,以乙腈-0.2%醋酸(85∶15)为流动相,体积流量为0.3 mL/min,紫外检测波长为205 nm。结果样品提取时间为10 min,待测物齐墩果酸和熊果酸达到基线分离,且无样品基质干扰,r大于0.999,平均回收率在95.8%～102.7%,本法测定结果与《中国药典》2015年版方法的平均质量分数差异不显著。结论本方法简便、快速,且一法多用,可用于8种中药中齐墩果酸和熊果酸的测定。
[Abstract]:Objective to establish a rapid analytical method for the simultaneous determination of Ligustrum lucidum, Prunella vulgaris, Hedyotis diffusa, Patrinia scabiosaefolia, loquat leaf, Eriobotrya japonica, Crataegus pinnatifida, a traditional Chinese medicinal material, of Verbena vulgaris Verbena officinalis, Ligustrum vulgaris and Prunella vulgaris,. The contents of oleanolic acid and ursolic acid in papaya Chaenomeles Fructus papaya. Methods the sample solution was prepared by rapid solvent extraction with methanol as extraction solvent and static extraction time of 6 min,. Acclaim C _ (30) column was used for liquid chromatography analysis, acetonitrile-0.2% acetic acid (85:15) was used as mobile phase. Volume flow rate 0.3 mL/min, UV detection wavelength 205 nm. Results the extraction time of Oleanolic acid and ursolic acid reached the baseline at 10 min, and there was no interference from sample matrix, r > 0.999.The average recovery was 95.8% and 102.7%, respectively. There was no significant difference between the results of this method and that of the 2015 edition of Chinese Pharmacopoeia. Conclusion the method is simple, rapid and multi-purpose. It can be used for the determination of oleanolic acid and ursolic acid in 8 kinds of traditional Chinese medicine.
【作者单位】：广西壮族自治区梧州食品药品检验所;广西梧州市人民医院药剂科;赛默飞世尔科技中国有限公司;
【分类号】：O657.72;R284.1

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