荧光光谱结合表面增强拉曼光谱法研究紫檀芪与人血清白蛋白相互作用
发布时间：2019-05-06 07:08
【摘要】：在模拟人体生理条件下,应用荧光光谱和表面增强拉曼光谱法研究了紫檀芪(PTE)与人血清白蛋白(HSA)之间相互作用机制。结果表明,HSA的荧光能被PTE静态猝灭,并伴随有非辐射能量转移作用,两者形成了1∶1复合物,结合距离r=1.495 nm,结合常数KA=1.12×104(298 K)、4.07×104(304 K)和2.45×105L/mol(310 K)。表面增强拉曼光谱研究揭示,PTE分子通过甲氧基与HSA进行结合;热力学数据表明,二者间的作用主要为疏水作用;标记竞争实验指出PTE优先结合HSA的位点Ⅲ。三维荧光光谱、同步荧光光谱和表面增强拉曼光谱结果显示,与PTE作用后,HSA构象发生变化,导致色氨酸残基周围环境疏水性降低,但对PTE分子构象影响不大。
[Abstract]:The interaction between (PTE) and human serum albumin (HSA) was studied by fluorescence spectroscopy and surface-enhanced Raman spectroscopy (SERS) under simulated physiological conditions of human body. The results showed that the fluorescence energy of HSA was quenched by PTE statically and accompanied by non-radiative energy transfer. The 1:1 complex was formed with the binding distance of 1.495 nm, binding constant KA=1.12 脳 10 4 (298 K),). 4.07 x 104 (304K) and 2.45 脳 105L/mol (310 K).) Surface-enhanced Raman spectroscopy (SERS) studies revealed that PTE molecules bind to HSA through methoxy groups; thermodynamic data show that the interaction between the two molecules is mainly hydrophobic; labeling competition experiments indicate that PTE binds HSA preferentially to site 鈪，
本文编号：2469978
[Abstract]:The interaction between (PTE) and human serum albumin (HSA) was studied by fluorescence spectroscopy and surface-enhanced Raman spectroscopy (SERS) under simulated physiological conditions of human body. The results showed that the fluorescence energy of HSA was quenched by PTE statically and accompanied by non-radiative energy transfer. The 1:1 complex was formed with the binding distance of 1.495 nm, binding constant KA=1.12 脳 10 4 (298 K),). 4.07 x 104 (304K) and 2.45 脳 105L/mol (310 K).) Surface-enhanced Raman spectroscopy (SERS) studies revealed that PTE molecules bind to HSA through methoxy groups; thermodynamic data show that the interaction between the two molecules is mainly hydrophobic; labeling competition experiments indicate that PTE binds HSA preferentially to site 鈪，
本文编号：2469978
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