HPLC法测定安神补脑液中防腐剂的含量

发布时间：2019-05-06 23:42

【摘要】：目的:建立测定安神补脑液中防腐剂含量的方法。方法:采用高效液相色谱法。色谱柱为Agilent Zorbax SB-C_(18),流动相为乙腈-0.05%磷酸溶液(梯度洗脱),流速为1.0 mL/min,检测波长为236 nm,柱温为30℃,进样量为10μL。结果:苯甲酸和羟苯乙酯检测质量浓度线性范围分别为12.85～128.54μg/mL(r=0.999 9)、4.05～40.48μg/mL(r=0.999 8);定量限分别为0.514、1.012μg/mL,检测限分别为0.171、0.353μg/mL;精密度、稳定性、重复性试验的RSD1.0%;加样回收率分别为99.38%～100.21%(RSD=0.31%,n=6)、97.96%～100.72%(RSD=0.91%,n=6)。结论:该方法操作简单、结果准确,可用于安神补脑液中防腐剂的含量测定。
[Abstract]:Objective: to establish a method for the determination of preservatives in Anshen Bunao liquid. Methods: high performance liquid chromatography (HPLC) was used. The chromatographic column was Agilent Zorbax SB-C_ (18), the mobile phase consisted of acetonitrile-0.05% phosphoric acid solution (gradient elution), the flow rate was 1.0 mL/min, the detection wavelength was 236nm, the column temperature was 30 鈩，

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