超高效液相色谱-串联质谱法测定保健食品中10种磷酸二酯酶-5抑制剂
发布时间：2019-05-22 01:40
【摘要】：建立了超高效液相色谱-串联质谱法(UPLC-MS/MS)同时测定保健食品中10种磷酸二酯酶-5(PDE-5)抑制剂。样品采用Waters Acquity UPLC BEH C18(100mm×2.1mm×1.7μm)色谱柱分离,以甲醇-0.1%甲酸水溶液为流动相进行梯度洗脱,甲醇为提取溶剂超声萃取,经HLB固相萃取柱净化浓缩;在电喷雾正离子模式下,以多反应监测(MRM)模式进行检测。结果表明,10种磷酸二酯酶-5抑制剂在1.0~100.0μg/L范围内线性关系良好,线性相关系数(R2)均大于0.993,方法定量限为1.0μg/kg;空白样品在100.0、200.0和1 000.0μg/kg添加水平下的回收率为85.6%~94.3%。该方法简便、快速、检出限低,适用于保健食品中10种磷酸二酯酶-5抑制剂的同时检测。
[Abstract]:An ultra-high performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) was established for the simultaneous determination of 10 phosphodiesterase-5 (PDE-5) inhibitors in health foods. The sample was separated by Waters Acquity UPLC BEH C18 (100mm 脳 2.1mm 脳 1.7 渭 m) column, gradient eluted with methanol-0.1% formic acid aqueous solution as mobile phase, ultrasonic extraction with methanol as extraction solvent, purified and concentrated by HLB solid phase extraction column. In the positive ion mode of electrospray, the detection was carried out by multi-reaction monitoring (MRM) mode. The results showed that the linear range of 10 phosphodiesterase-5 inhibitors was 1.0 ~ 100.0 渭 g / L, the linear correlation coefficient (R2) was more than 0.993, and the quantitative limit of the method was 1.0 渭 g / kg;. The recovery of blank sample at 100.0200.0 and 1000.0 渭 g / kg was 85.6% 鈮，
本文编号：2482566
[Abstract]:An ultra-high performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) was established for the simultaneous determination of 10 phosphodiesterase-5 (PDE-5) inhibitors in health foods. The sample was separated by Waters Acquity UPLC BEH C18 (100mm 脳 2.1mm 脳 1.7 渭 m) column, gradient eluted with methanol-0.1% formic acid aqueous solution as mobile phase, ultrasonic extraction with methanol as extraction solvent, purified and concentrated by HLB solid phase extraction column. In the positive ion mode of electrospray, the detection was carried out by multi-reaction monitoring (MRM) mode. The results showed that the linear range of 10 phosphodiesterase-5 inhibitors was 1.0 ~ 100.0 渭 g / L, the linear correlation coefficient (R2) was more than 0.993, and the quantitative limit of the method was 1.0 渭 g / kg;. The recovery of blank sample at 100.0200.0 and 1000.0 渭 g / kg was 85.6% 鈮，
本文编号：2482566
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