基于三维有序多孔材料修饰电极制备及用于药物分子检测研究
发布时间：2019-05-28 19:21
【摘要】：电化学传感器因其分析速度快、成本低、操作简便及易于实现微型化等特点,在分析化学、食品安全、环境监测、临床医学和新药筛选等领域有着重要的应用价值。纳米材料具有比表面积大、导电性好、优良的催化特性和生物相容性,可以有效改善传感器的分析性能,因此被广泛应用于构建电化学传感器的修饰材料。本论文基于三维有序多孔材料,分别制备了三种修饰电极,并将其应用于药物分子的电化学研究及定量检测。主要研究结果如下:(1)采用模板法制备三维有序金掺杂纳米TiO2多孔结构的修饰电极(3DOM/ITO),并在该修饰电极表面固定抗菌素卡那霉素(Kana)的适配体(aptamer),从而构建一种新型的适配体生物传感器(Kana-aptamer/3DOM/ITO)。利用透射电镜(TEM)、扫描电镜(SEM)、循环伏安法(CV)、电化学阻抗谱(EIS)等方法对修饰电极的形貌和电化学性质进行表征。在优化条件下,将该修饰电极应用于牛奶中Kana残留量的检测。Kana的浓度与Fe(CN)63-/4-在适配体修饰电极上的氧化峰电流降低值与成正相关,其测定线性范围为8.6×10-7～1.7×10-4molL-1 检出限为 7.0×10-8molL-1。(2)在3DOM/ITO电极表面通过电化学聚合制备木犀草素(Lu)印迹电极(MIP/3DOM/ITO)。采用SEM、CV、EIS及差分脉冲伏安法(DPV)等表征电极表面形貌及电化学性质。讨论支持电解质pH、电聚合圈数、模板分子洗脱条件及富集时间等因素对印迹电极分析性能及Lu电流响应的影响。在优化实验条件下,Lu的氧化峰电流与其浓度在5×10-8～3×10-5molL-1范围内呈线性关系,检测限为2.4×10-8 molL-1(S/N=3)。此外,将该印迹电极成功用于定量测定中成药独一味胶囊中Lu的含量。(3)通过水热法制备纳米级金属-有机骨架材料(MOF) Nano-PCN-222,以Nano-PCN-222作为修饰材料构建了一种新的MOF电化学传感器,并用于氨茶碱(AP)的定量检测。AP在该修饰电极上有一较强氧化峰,且电流强度明显高于玻碳电极,表明Nano-PCN-222能有效地促进氨茶碱在电极表面的电化学反应,从而提高氨茶碱在修饰电极上的电化学响应及检测灵敏度。采用SEM、X-射线衍射(XRD)等手段对制备的MOF材料进行了结构和形貌表征。讨论了实验条件,如:Nano-PCN-222固载量、支持电解质、溶液pH、模板分子富集时间等对氨茶碱测定的影响。在优化条件下,检测氨茶碱的浓度线性范围为5.0×10-7～1.5×10-4molL-1,检出限为6.0×10-8mol L-1 (S/N=3),并实现了对氨茶碱注射液和临床血样中氨茶碱的定量检测。
[Abstract]:Electrochemical sensors have important application value in analytical chemistry, food safety, environmental monitoring, clinical medicine and new drug screening because of their fast analysis speed, low cost, simple operation and easy miniaturization. Nanomaterials have large specific surface area, good conductivity, excellent catalytic properties and biocompatibility, and can effectively improve the analytical performance of the sensor, so they are widely used in the construction of electrochemical sensor modification materials. In this paper, three kinds of modified electrodes were prepared based on three-dimensional ordered porous materials and applied to the electrochemical study and quantitative detection of drug molecules. The main results are as follows: (1) the modified electrode (3DOM/ITO) with three-dimensional ordered gold-doped nano-TiO2 porous structure was prepared by template method, and the aptamer (aptamer), of antibiotic kanamycin (Kana) was fixed on the surface of the modified electrode. A new type of adaptive biosensor (Kana-aptamer/3DOM/ITO) was constructed. The morphology and electrochemical properties of the modified electrode were characterized by transmission electron microscope (TEM),) scanning electron microscope (SEM),) cyclic Voltammetric method (CV),) electrochemical impedance spectroscopy (EIS). Under the optimized conditions, the modified electrode was applied to the determination of Kana residues in milk. The concentration of Kana was positively correlated with the decrease of oxidation peak current of Fe (CN) 63 鈮，
本文编号：2487303
[Abstract]:Electrochemical sensors have important application value in analytical chemistry, food safety, environmental monitoring, clinical medicine and new drug screening because of their fast analysis speed, low cost, simple operation and easy miniaturization. Nanomaterials have large specific surface area, good conductivity, excellent catalytic properties and biocompatibility, and can effectively improve the analytical performance of the sensor, so they are widely used in the construction of electrochemical sensor modification materials. In this paper, three kinds of modified electrodes were prepared based on three-dimensional ordered porous materials and applied to the electrochemical study and quantitative detection of drug molecules. The main results are as follows: (1) the modified electrode (3DOM/ITO) with three-dimensional ordered gold-doped nano-TiO2 porous structure was prepared by template method, and the aptamer (aptamer), of antibiotic kanamycin (Kana) was fixed on the surface of the modified electrode. A new type of adaptive biosensor (Kana-aptamer/3DOM/ITO) was constructed. The morphology and electrochemical properties of the modified electrode were characterized by transmission electron microscope (TEM),) scanning electron microscope (SEM),) cyclic Voltammetric method (CV),) electrochemical impedance spectroscopy (EIS). Under the optimized conditions, the modified electrode was applied to the determination of Kana residues in milk. The concentration of Kana was positively correlated with the decrease of oxidation peak current of Fe (CN) 63 鈮，
本文编号：2487303
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