用高效液相色谱组方指纹图谱智能预测中药质量的新模式
发布时间：2019-06-15 16:33
【摘要】：建立大黄、黄芩、黄连的高效液相色谱(HPLC)组方指纹图谱,确定其融合模型,观察组方融合指纹图谱(CSF)与一清片复方样品指纹图谱的一致性,从而以CSF代替复方整体来智能预测复方制剂质量。用二极管阵列检测器(DAD)同时测定黄芩、大黄、黄连和一清片在268 nm波长下的HPLC指纹图谱,并使用系统指纹定量法进行定性、定量评价。结果 CSF涵盖各单味药主要色谱峰信息,即CSF共有峰(55个)涵盖一清片样品共有峰(50个)的主要指纹图谱信息。15批样品的质量除YQT-S01为5级外,其他质量均为3级及以上。各组合模式CSF质量除CSF-2为6级外,其余均为2级或1级。该文探讨了标准指纹图谱和中药组方融合指纹图谱的相关性,可以与相应的计算机评价软件相结合,通过组方融合指纹图谱所代表复方制剂的整体指纹图谱来实现智能预测中药成方制剂质量的新模式。
[Abstract]:A high-performance liquid chromatography (HPLC) formula of Radix et Rhizoma Rhei, Radix et Rhizoma Rhei, and Rhizoma Coptidis is established to obtain a fingerprint, and the fusion model is determined, and the consistency of the combination fingerprint (CSF) of the formula and the fingerprint of a clear tablet compound sample is observed, so that the quality of the compound preparation can be intelligently predicted by using the CSF instead of the compound whole. The HPLC fingerprint of the yellow, rhubarb, coptis and a clear slice at the wavelength of 268 nm was also determined by a diode array detector (DAD), and the qualitative and quantitative evaluation was carried out using the system fingerprint quantitative method. Results CSF covers the main peak information of the single-flavor drug, that is, the common peak (55) of the CSF covers the main fingerprint information of the common peak (50) of a clear-plate sample. The quality of the 15-batch samples, except that YQT-S01 is 5, the other quality is Grade 3 and above. The CSF-2 of each combined mode was grade 2 or grade 1 except for CSF-2. In this paper, the correlation of the standard fingerprint and the fingerprint of the traditional Chinese medicine formula is discussed, and the new model of the quality of the traditional Chinese medicine preparation can be realized by the combination of the corresponding computer evaluation software and the whole fingerprint of the compound preparation represented by the combination of the combination of the prescription and the fingerprint.
【作者单位】： 沈阳药科大学药学院;暨南大学药学院中药及天然药物研究所;
【基金】：国家自然科学基金人才培养基金项目(J1103606)~~
【分类号】：O657.72;R284
[Abstract]:A high-performance liquid chromatography (HPLC) formula of Radix et Rhizoma Rhei, Radix et Rhizoma Rhei, and Rhizoma Coptidis is established to obtain a fingerprint, and the fusion model is determined, and the consistency of the combination fingerprint (CSF) of the formula and the fingerprint of a clear tablet compound sample is observed, so that the quality of the compound preparation can be intelligently predicted by using the CSF instead of the compound whole. The HPLC fingerprint of the yellow, rhubarb, coptis and a clear slice at the wavelength of 268 nm was also determined by a diode array detector (DAD), and the qualitative and quantitative evaluation was carried out using the system fingerprint quantitative method. Results CSF covers the main peak information of the single-flavor drug, that is, the common peak (55) of the CSF covers the main fingerprint information of the common peak (50) of a clear-plate sample. The quality of the 15-batch samples, except that YQT-S01 is 5, the other quality is Grade 3 and above. The CSF-2 of each combined mode was grade 2 or grade 1 except for CSF-2. In this paper, the correlation of the standard fingerprint and the fingerprint of the traditional Chinese medicine formula is discussed, and the new model of the quality of the traditional Chinese medicine preparation can be realized by the combination of the corresponding computer evaluation software and the whole fingerprint of the compound preparation represented by the combination of the combination of the prescription and the fingerprint.
【作者单位】： 沈阳药科大学药学院;暨南大学药学院中药及天然药物研究所;
【基金】：国家自然科学基金人才培养基金项目(J1103606)~~
【分类号】：O657.72;R284
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