基于光强传输方程相位成像的宽场相干反斯托克斯拉曼散射显微背景抑制
发布时间：2019-07-08 15:07
【摘要】：相干反斯托克斯拉曼散射(CARS)显微能够对样品的特殊化学组分进行选择性成像,无需荧光标记,在生物医学领域被广泛应用.然而,传统的CARS图像往往存在非共振背景信号.本文将基于光强传输方程的单光束相位成像技术用于CARS显微成像,来抑制CARS的非共振背景信号.该方法通过记录样品在三个相邻平面上的CARS图像,然后利用光强传输方程获取CARS光场的相位分布,最后利用共振CARS信号和非共振背景信号在相位上的差异,实现了对背景噪声的抑制.该方法无需参考光,通过三次测量可完成CARS的背景噪声抑制,具有良好的应用前景.
[Abstract]:Coherent anti-Stoke Slaman scattering (CARS) microscope can selectively image the special chemical components of samples without fluorescence labeling and is widely used in biomedical field. However, there are often non-resonant background signals in traditional CARS images. In this paper, a single beam phase imaging technique based on light intensity propagation equation is applied to CARS microscopic imaging to suppress the non-resonant background signal of CARS. In this method, the CARS images of the samples on three adjacent planes are recorded, and then the phase distribution of the CARS light field is obtained by using the light intensity transmission equation. Finally, the background noise is suppressed by using the phase difference between the resonance CARS signal and the non-resonance background signal. This method does not need reference light, and the background noise suppression of CARS can be completed by three measurements, and it has a good application prospect.
【作者单位】： 西安电子科技大学物理与光电工程学院;中国科学院西安光学精密机械研究所瞬态光学与光子技术国家重点实验室;
【基金】：国家自然科学基金(批准号:61605150,61475187,61575154,61377008) 中央高校基本科研业务费专项资金(批准号:JB160511,XJS16005,JBG160502) the “Thüringer Ministerium für Bildung,Wissenschaft und Kultur”(TMBWK,projects:B578-06001,14.90HWP,B714-07037) and the “Carl Zeiss Stiftung”and the Federal Ministry of Education and Research,Germany(FKZ:13N10508)资助的课题~~
【分类号】：O657.37
本文编号：2511675
[Abstract]:Coherent anti-Stoke Slaman scattering (CARS) microscope can selectively image the special chemical components of samples without fluorescence labeling and is widely used in biomedical field. However, there are often non-resonant background signals in traditional CARS images. In this paper, a single beam phase imaging technique based on light intensity propagation equation is applied to CARS microscopic imaging to suppress the non-resonant background signal of CARS. In this method, the CARS images of the samples on three adjacent planes are recorded, and then the phase distribution of the CARS light field is obtained by using the light intensity transmission equation. Finally, the background noise is suppressed by using the phase difference between the resonance CARS signal and the non-resonance background signal. This method does not need reference light, and the background noise suppression of CARS can be completed by three measurements, and it has a good application prospect.
【作者单位】： 西安电子科技大学物理与光电工程学院;中国科学院西安光学精密机械研究所瞬态光学与光子技术国家重点实验室;
【基金】：国家自然科学基金(批准号:61605150,61475187,61575154,61377008) 中央高校基本科研业务费专项资金(批准号:JB160511,XJS16005,JBG160502) the “Thüringer Ministerium für Bildung,Wissenschaft und Kultur”(TMBWK,projects:B578-06001,14.90HWP,B714-07037) and the “Carl Zeiss Stiftung”and the Federal Ministry of Education and Research,Germany(FKZ:13N10508)资助的课题~~
【分类号】：O657.37
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