金磁纳米颗粒的制备及其在表面增强拉曼光谱快速检测黄曲霉毒素B1中的应用
发布时间：2019-07-10 11:40
【摘要】：本文采用纳米Fe_3O_4颗粒作为磁性核心,先用四乙氧基硅烷、再用3-巯丙基三乙氧基硅烷和3-氨丙基三乙氧基硅烷修饰Fe_3O_4颗粒,形成表面带-NH_2和-SH的Fe_3O_4/SiO_2纳米颗粒,进一步通过-NH_2的静电吸附和Au-S键的作用将金纳米颗粒组装在Fe_3O_4/SiO_2表面,形成具有核壳结构的Fe_3O_4/SiO_2/Au金磁纳米颗粒,并用透射电子显微镜镜(TEM)、能量色散X射线光谱仪(EDX)、紫外可见分光光度计(UV-vis)等技术对金磁纳米颗粒进行了形貌观测及性质表征。利用Fe_3O_4/SiO_2/Au金磁纳米颗粒作为拉曼活性基底,用表面增强拉曼光谱仪对黄曲霉毒素B1(AFB1)进行直接快速检测,发现无外磁体浓缩的情况下AFB1的检测限大于10.0μg/m L,在外磁体浓缩金磁纳米颗粒的情况下检测限降低100倍(≤0.1μg/m L),检测线性范围0.1μg/m L~10.0μg/m L,检测的样品回收率为84.35%~91.98%,相对标准偏差在4.88%~9.90%之间。
[Abstract]:In this paper, nano-Fe_3O_4 particles were used as magnetic core, first with tetraethoxysilane, then modified with 3-mercaptopropyltriethoxysilane and 3-aminopropyl triethoxysilane to form Fe_3O_4/SiO_2 nanoparticles with-NH_2 and-SH on the surface. Furthermore, the gold nanoparticles were assembled on the surface of Fe_3O_4/SiO_2 by electrostatic adsorption of-NH_2 and Au-S bond, and Fe_3O_4/SiO_2/Au gold magnetic nanoparticles with core-shell structure were formed. The (TEM), energy dispersive X-ray spectrometer (EDX), was used as transmission electron microscope. The morphology and properties of gold magnetic nanoparticles were observed by UV-vis spectrophotometer (UV-vis). Using Fe_3O_4/SiO_2/Au gold magnetic nanoparticles as Raman active substrate, aflatoxin B1 (AFB1) was detected directly and rapidly by surface enhanced Raman spectrometer. It was found that the detection limit of AFB1 was more than 10.0 渭 g / mL in the absence of external magnet concentration, and the detection limit was reduced by 100 times (鈮，
本文编号：2512577
[Abstract]:In this paper, nano-Fe_3O_4 particles were used as magnetic core, first with tetraethoxysilane, then modified with 3-mercaptopropyltriethoxysilane and 3-aminopropyl triethoxysilane to form Fe_3O_4/SiO_2 nanoparticles with-NH_2 and-SH on the surface. Furthermore, the gold nanoparticles were assembled on the surface of Fe_3O_4/SiO_2 by electrostatic adsorption of-NH_2 and Au-S bond, and Fe_3O_4/SiO_2/Au gold magnetic nanoparticles with core-shell structure were formed. The (TEM), energy dispersive X-ray spectrometer (EDX), was used as transmission electron microscope. The morphology and properties of gold magnetic nanoparticles were observed by UV-vis spectrophotometer (UV-vis). Using Fe_3O_4/SiO_2/Au gold magnetic nanoparticles as Raman active substrate, aflatoxin B1 (AFB1) was detected directly and rapidly by surface enhanced Raman spectrometer. It was found that the detection limit of AFB1 was more than 10.0 渭 g / mL in the absence of external magnet concentration, and the detection limit was reduced by 100 times (鈮，
本文编号：2512577
本文链接：https://www.wllwen.com/kejilunwen/huaxue/2512577.html
教材专著
