合成具有优良光学性能的多维多层上转换纳米粒子及生物分析中的应用
发布时间:2021-11-11 13:57
在本论文中,我们设计了具有不同形状、结构和表面性质的上转换纳米粒子,应用于构建生物传感器和上转换荧光成像,具体研究成果如下:1.合成了具有三维上转换核-双壳纳米粒子(UCNDs),包括一个过渡层(NaYF4:Yb,Er,Ca)包覆的活性核(NaYF4:Yb,Ca)和一个活性外壳(NaNdF4:Yb,Ca)。外枝晶壳中高浓度的Nd3+致敏剂增强了发光强度,而富含Yb3+的过渡层在外壳与内核之间起到了有效的能量迁移作用,防止了 Nd3+造成的不正常的猝灭效应。这些独特的结构和成分优势使其在980 nm激发下的上转换发光(UCL)分别比 NaYF4:Yb,Er,Ca@NaYF4:Yb,Ca 核壳 UCNPs 和NaYF4:Yb,Er,Ca 球形核 UCNPs 提高了5倍和 15倍。SiO2-COOH 涂层的 UCNDs(UCND@SiO2-COOH)作为高效的长期发光探针被成功地用于体外和体内生物成像,且无明显毒性。UCND@SiO2-COOH的摄取和滞留几乎在肝脏和脾脏中。本研究可能为制备具有生物医学应用价值的三维UCNDs纳米结构开辟道路。2.开发了一种简便的可扩展方法,用于理性设计多维...
【文章来源】:中国科学技术大学安徽省 211工程院校 985工程院校
【文章页数】:109 页
【学位级别】:博士
【文章目录】:
摘要
Abstract
Chapter 1:Introduction
1.1 Upconversion phenomenon and upconversion nanoparticles
1.1.1 Upconversion phenomenon
1.1.2 Upconversion Mechanisms
1.1.2.1 Excited-state absorption (ESA)
1.1.2.2 Energy Transfer Upconversion (ETU)
1.1.2.3 Cooperative Sensitization Upconversion (CSU)
1.1.2.4 Cooperative upconversion (CU)
1.1.2.5 The Cross relaxation (CR)
1.1.2.6 Photon Avalanche (PA)
1.1.2.7 Energy Migration Upconversion (EMU)
1.1.2.8 Energy cascaded upconversion (ECU)
1.1.3 Synthetic methods of upconversion nanoparticles
1.1.3.1 Hydro (Solvo) thermal Synthesis
1.1.3.2 Thermal Decomposition Synthesis
1.1.4 Surface functionalization of hydrophobic UCNPs
1.1.5 The Bioanalytical and Biomedical Applications of UCNPs
1.1.6 Core-shell, Core-double-shell upconversion Nanostructures
1.1.7 Investigation of the multidimensional and multilayered upconversionNanoarchitectures
1.1.7.1 Morphology control of the multidimensional and multilayeredupconversion Nanoarchitectures
1.1.8 Absorption of UCNPs
1.1.9 The novelty of the work
Chapter 2:Rational Synthesis of three-dimensional Core-double ShellUpconversion Nanodendrites with Ultrabright Luminescence for Bioimaging Application
2.1 Abstract
2.2 Introduction
2.3 Experimental
2.3.1 Chemicals and Materials
2.3.2 Materials Characterization
2.3.3 Synthesis of UCNDs
2.3.4 Synthesis of UCND@SiO_2-COOH
2.3.5 Cytotoxicity Study of UCND@SiO_2-COOH
2.3.6 UCL Imaging of Cells
2.3.7 In vivo UCL Imaging of UCND@SiO_2-COOH
2.3.8 In Vivo Toxicology Analysis of UCND@SiO_2-COOH
2.4 Results and discussion
2.5 Conclusion
Chapter 3:Smart Design of Exquisite Multidimensional Multilayered Sand-clock-like Upconversion Nanostructures with Ultrabright Luminescenceas Efficient Luminescence Probes for Bioimaging Application
3.1 Abstract
3.2 Introduction
3.3 Experimental section
3.3.1 Materials
3.3.2 Characterization
3.3.3 Synthesis of UCNPs core
3.3.4 Synthesis of UCNPs core-shell
3.3.5 Synthesis of multidimensional UCCKs
3.3.6 Synthesis of UCCK@SiO_2-COOH
3.3.7 Measurement of Upconversion Luminescence Quantum Yields
3.3.8 Cytotoxicity Study of UCCK@SiO_2-COOH
3.3.9 In vitro experiments
3.3.10 Surface modification of UCCK@SiO_2-COON with folic acid (FA)
3.3.11 UCCK@SiO_2-FA specific targeting ability study
3.4 Results and discussion
3.4.1 Fabrication of the multidimensional UCCKs
3.4.2 Growth mechanism of the multidimensional UCCKs
3.4.3 Characterization of the multidimensional UCCKs
3.4.4 Upconversion luminescence of the multidimensional UCCKs
3.4.5 Cell imaging
3.5 Conclusion
Chapter 4: Conclusion and Prospectives
4.1 Conclusion
4.2 Prospectives
References
Acknowledgements
Curriculum vitae
List of Publications
本文编号:3488979
【文章来源】:中国科学技术大学安徽省 211工程院校 985工程院校
【文章页数】:109 页
【学位级别】:博士
【文章目录】:
摘要
Abstract
Chapter 1:Introduction
1.1 Upconversion phenomenon and upconversion nanoparticles
1.1.1 Upconversion phenomenon
1.1.2 Upconversion Mechanisms
1.1.2.1 Excited-state absorption (ESA)
1.1.2.2 Energy Transfer Upconversion (ETU)
1.1.2.3 Cooperative Sensitization Upconversion (CSU)
1.1.2.4 Cooperative upconversion (CU)
1.1.2.5 The Cross relaxation (CR)
1.1.2.6 Photon Avalanche (PA)
1.1.2.7 Energy Migration Upconversion (EMU)
1.1.2.8 Energy cascaded upconversion (ECU)
1.1.3 Synthetic methods of upconversion nanoparticles
1.1.3.1 Hydro (Solvo) thermal Synthesis
1.1.3.2 Thermal Decomposition Synthesis
1.1.4 Surface functionalization of hydrophobic UCNPs
1.1.5 The Bioanalytical and Biomedical Applications of UCNPs
1.1.6 Core-shell, Core-double-shell upconversion Nanostructures
1.1.7 Investigation of the multidimensional and multilayered upconversionNanoarchitectures
1.1.7.1 Morphology control of the multidimensional and multilayeredupconversion Nanoarchitectures
1.1.8 Absorption of UCNPs
1.1.9 The novelty of the work
Chapter 2:Rational Synthesis of three-dimensional Core-double ShellUpconversion Nanodendrites with Ultrabright Luminescence for Bioimaging Application
2.1 Abstract
2.2 Introduction
2.3 Experimental
2.3.1 Chemicals and Materials
2.3.2 Materials Characterization
2.3.3 Synthesis of UCNDs
2.3.4 Synthesis of UCND@SiO_2-COOH
2.3.5 Cytotoxicity Study of UCND@SiO_2-COOH
2.3.6 UCL Imaging of Cells
2.3.7 In vivo UCL Imaging of UCND@SiO_2-COOH
2.3.8 In Vivo Toxicology Analysis of UCND@SiO_2-COOH
2.4 Results and discussion
2.5 Conclusion
Chapter 3:Smart Design of Exquisite Multidimensional Multilayered Sand-clock-like Upconversion Nanostructures with Ultrabright Luminescenceas Efficient Luminescence Probes for Bioimaging Application
3.1 Abstract
3.2 Introduction
3.3 Experimental section
3.3.1 Materials
3.3.2 Characterization
3.3.3 Synthesis of UCNPs core
3.3.4 Synthesis of UCNPs core-shell
3.3.5 Synthesis of multidimensional UCCKs
3.3.6 Synthesis of UCCK@SiO_2-COOH
3.3.7 Measurement of Upconversion Luminescence Quantum Yields
3.3.8 Cytotoxicity Study of UCCK@SiO_2-COOH
3.3.9 In vitro experiments
3.3.10 Surface modification of UCCK@SiO_2-COON with folic acid (FA)
3.3.11 UCCK@SiO_2-FA specific targeting ability study
3.4 Results and discussion
3.4.1 Fabrication of the multidimensional UCCKs
3.4.2 Growth mechanism of the multidimensional UCCKs
3.4.3 Characterization of the multidimensional UCCKs
3.4.4 Upconversion luminescence of the multidimensional UCCKs
3.4.5 Cell imaging
3.5 Conclusion
Chapter 4: Conclusion and Prospectives
4.1 Conclusion
4.2 Prospectives
References
Acknowledgements
Curriculum vitae
List of Publications
本文编号:3488979
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