整体柱电渗泵—毛细管离子色谱联用技术研究
发布时间：2018-09-17 07:30
【摘要】：微流体的驱动与控制是微流动分析系统中一个关键课题。在诸多微型泵中,电渗泵(EOP)是利用电渗流实现液体的驱动,具有易于制作、无活动活塞、流量稳定、输出压力高等优点,受到越来越多的关注。本论文旨在发展电渗泵泵体制作技术及相应的色谱接口应用于色谱系统。 发展了一种简便、快捷的新型硅胶凝胶柱制备方法并将其用于电渗泵；硅胶整体柱整个制备过程少于4 h,所制备整体柱具有高柱压降、高电阻等优势,非常适合用于电渗泵；对该电渗泵性能进行了系列评价,包括电流、流速、压力等参数与施加电压之间的关系等,证明其具有良好的性能。 为提高电渗泵的输出压力,提出一种新型的级联电渗泵：首先通过优化单体、致孔剂的比例等条件分别制作出阴离子型和阳离子型毛细管整体柱；将阴、阳离子型毛细管整体柱交替连接即可组合成一新型级联电渗泵。利用阴阳离子型毛细管整体柱表面电荷的差异可实现级联电渗泵施加电场方向的自然切换,避免了常规级联电渗泵施加电场不易切换的弊端；并对该级联电渗泵进行性能评价。 将硅胶整体柱电渗泵结合微型淋洗液发生器应用于毛细管离子色谱,考察了联用装置的稳定性,并成功应用于常规阴离子分析并得到了有效分离。该思路既解决了电渗泵驱动纯水在色谱中的应用问题,亦可为毛细管离子色谱提供了一种新型的微泵系统。
[Abstract]:The drive and control of micro-fluid is a key issue in micro-flow analysis system. Electroosmotic pump (EOP) is widely used to drive liquid by electroosmotic flow (EOF). It has many advantages such as easy to manufacture, no moving piston, stable flow rate and high output pressure, which has attracted more and more attention. The purpose of this paper is to develop the manufacture technology of electroosmotic pump and the application of the corresponding chromatographic interface to the chromatographic system. A simple and fast preparation method of silica gel column was developed and used in electroosmotic pump, the whole preparation process of silica gel whole column was less than 4 h, the prepared monolithic column had the advantages of high column pressure drop and high resistance, so it was very suitable for electroosmotic pump. The performance of the electroosmotic pump is evaluated, including the relationship between current, flow rate, pressure and applied voltage. It is proved that the pump has good performance. In order to improve the output pressure of electroosmotic pump, a new type of combined electroosmotic pump was proposed. Firstly, anion and cationic capillary monolithic columns were made by optimizing monomer and pore-forming agent ratio. Cationic capillary column can be connected alternately to form a new type of combined electroosmotic pump. By using the difference of surface charge on the monolithic column of anion capillary tube, the electric field direction of the combined electroosmotic pump can be switched naturally, which avoids the disadvantage that it is difficult to change the electric field applied by the conventional electroosmotic pump, and the performance of the combined electroosmotic pump is evaluated. The silica gel monolithic column electroosmotic pump combined with a micro eluant generator was applied to capillary ion chromatography. The stability of the combined device was investigated and successfully applied to the routine anion analysis and effective separation was obtained. This idea not only solves the application problem of pure water driven by electroosmotic pump in chromatography, but also provides a new micro-pump system for capillary ion chromatography.
【学位授予单位】：华东理工大学
【学位级别】：硕士
【学位授予年份】：2011
【分类号】：TH38
本文编号：2245167
[Abstract]:The drive and control of micro-fluid is a key issue in micro-flow analysis system. Electroosmotic pump (EOP) is widely used to drive liquid by electroosmotic flow (EOF). It has many advantages such as easy to manufacture, no moving piston, stable flow rate and high output pressure, which has attracted more and more attention. The purpose of this paper is to develop the manufacture technology of electroosmotic pump and the application of the corresponding chromatographic interface to the chromatographic system. A simple and fast preparation method of silica gel column was developed and used in electroosmotic pump, the whole preparation process of silica gel whole column was less than 4 h, the prepared monolithic column had the advantages of high column pressure drop and high resistance, so it was very suitable for electroosmotic pump. The performance of the electroosmotic pump is evaluated, including the relationship between current, flow rate, pressure and applied voltage. It is proved that the pump has good performance. In order to improve the output pressure of electroosmotic pump, a new type of combined electroosmotic pump was proposed. Firstly, anion and cationic capillary monolithic columns were made by optimizing monomer and pore-forming agent ratio. Cationic capillary column can be connected alternately to form a new type of combined electroosmotic pump. By using the difference of surface charge on the monolithic column of anion capillary tube, the electric field direction of the combined electroosmotic pump can be switched naturally, which avoids the disadvantage that it is difficult to change the electric field applied by the conventional electroosmotic pump, and the performance of the combined electroosmotic pump is evaluated. The silica gel monolithic column electroosmotic pump combined with a micro eluant generator was applied to capillary ion chromatography. The stability of the combined device was investigated and successfully applied to the routine anion analysis and effective separation was obtained. This idea not only solves the application problem of pure water driven by electroosmotic pump in chromatography, but also provides a new micro-pump system for capillary ion chromatography.
【学位授予单位】：华东理工大学
【学位级别】：硕士
【学位授予年份】：2011
【分类号】：TH38
【参考文献】
相关期刊论文 前3条
1 谭峰;杨丙成;关亚风;;小型低压电渗输液泵的研究[J];分析化学;2006年02期
2 ;Electroosmotic Driving Liquid Using Nanosilica Packed Column[J];Chinese Chemical Letters;2005年06期
3 尹俊发;魏晓奕;杨更亮;;有机聚合物整体柱的制备与应用的研究进展[J];色谱;2007年02期
，本文编号：2245167
本文链接：https://www.wllwen.com/kejilunwen/jixiegongcheng/2245167.html
