山西临汾褐煤微生物降解工艺条件的优化

发布时间：2018-03-24 04:18

本文选题：临汾褐煤　切入点：细菌　出处：《煤炭转化》2017年02期

【摘要】：对6株细菌和硝酸氧化的山西临汾褐煤进行菌-煤匹配降解实验,筛选出降解优势菌恶臭假单胞菌.在单因素试验的基础上,通过正交试验优化了恶臭假单胞菌降解临汾褐煤的工艺条件,得出的影响权重为:煤样粒度煤浆质量浓度培养时间接种量.较优降解工艺条件为:煤样粒度75μm,煤浆质量浓度0.200 0g/10mL,培养时间14d,接种量1.0mL/10mL.此条件下的降解转化率达到46.81%.对降解后的煤残渣和降解产物进行红外检测,结果表明:煤残渣在氧化煤既有波数处的吸收大部分已消失或者变弱,说明煤中的有机大分子已被恶臭假单胞菌降解成小分子,包含在降解后的黑色油状滤液中.滤液分级萃取物的GC-MS总离子流色谱证明了降解液中含有丰富的小分子有机物,且大多为脂肪烃、酯、羧酸及芳香族化合物.
[Abstract]:Six strains of bacteria and nitric acid oxidized lignite in Linfen, Shanxi Province, were tested by bacterial-coal matching degradation test, and the dominant bacteria, Pseudomonas odour, were screened out. The technological conditions for degradation of Linfen lignite by Pseudomonas odour were optimized by orthogonal test. The optimum degradation conditions are as follows: coal sample size 75 渭 m, coal slurry concentration 0.200 g / 10 mL, culture time 14 days, inoculation amount 1.0 mL / 10 mL. To 46.81. the degradation of coal residue and degradation products were detected by infrared, The results show that most of the absorption of coal residues in oxidized coal has disappeared or weakened, indicating that the organic macromolecules in coal have been degraded into small molecules by Pseudomonas odour. The GC-MS total ion flow chromatography of the fractionated extract of the filtrate shows that there are abundant small molecular organic compounds in the degradation solution, and most of them are aliphatic hydrocarbons, esters, carboxylic acids and aromatic compounds.
【作者单位】：西安科技大学化学与化工学院;商洛学院化学工程与现代材料学院;
【基金】：国家自然科学基金资助项目(21373158) 陕西科技厅中澳合作项目(2013KW17)
【分类号】：Q949.97;TD849.2

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