微生物脱除云南烟煤有机硫研究

发布时间：2018-06-04 06:17

本文选题：煤炭 + 二苯并噻吩　；参考：《昆明理工大学》2015年硕士论文

【摘要】：高硫煤炭在燃烧过程中产生大量的SO2气体,严重污染环境,虽然用物理和化学法能将高硫煤进行脱硫处理,但从环境友好和经济效益来看,它们并不是最好的选择。利用微生物技术降低煤炭中有机硫含量,反应条件温和、对环境友好,因此开展微生物煤炭脱硫技术的研究与开发对煤炭产业发展意义重大。本论文从云南省的圭山、小龙潭和宜良大桥沟煤矿矿区采集了不同的煤炭样品,同时以污水处理厂活性污泥为起始菌的来源。首先利用活性污泥中的混合菌处理煤炭样品,在处理过程中对培养基、培养温度、pH等都进行了优化,但始终没有看到明显的有机硫降解效果。随后利用DBT为底物,利用无限稀释法和选择性培养基分离得到了8株细菌,对8株菌进行了形态学观察及16S rRNA基因测序,根据16S rRNA基因序列比对结果将其初步鉴定为：根瘤菌属(Rhizobiumsp.)、黄色杆菌属(Xanthobacter sp.)、红串红球菌(Rhodococcus erythropolis)、红球菌属(Rhodococcus sp.)、假单胞菌属(Pseudomonas sp.)、节杆菌属(Arthrobacter sp.)和莫拉克斯氏菌属(Moraxella sp.)的菌株。以DBT为底物,利用高效液相色谱法对这8株菌的降解能力进行了评估,发现6株能够降解DBT(Dibenzothiophene),其中HWBHT2菌培养3d后的DBT降解率为45.8%；XFJ2菌培养7d后DBT的降解率为76.2%。随后利用菌株XFJ2和HWBHT2对云南烟煤进行了脱有机硫的处理,处理结果委托云南省煤炭产品质量监督检验站进行了检测,但发现这两株菌对云南烟煤中的有机硫不能降解,尽管对培养基和生理生化条件的进行了改进和优化,依然未能降低煤中的有机硫含量。究其原因：(1)云南烟煤中的有机硫可能并非DBT类物质；(2)云南烟煤中的其它成分对细菌的生长和代谢具有抑制作用。本研究共分离得到6株能够降解DBT的细菌,并研究了其降解DBT的能力,为进一步开展煤炭脱除有机硫的实际应用提供了一定的理论和实践依据,但利用微生物脱除煤炭中的有机硫还有待进一步研究。
[Abstract]:High sulfur coal produces a large amount of SO2 gas in the combustion process, which pollutes the environment seriously. Although it can be desulphurized by physical and chemical methods, it is not the best choice from the point of view of environmental friendliness and economic benefit. Using microbial technology to reduce the content of organic sulfur in coal, the reaction conditions are mild and friendly to the environment. Therefore, it is of great significance to develop microbial coal desulphurization technology for the development of coal industry. In this paper, different coal samples were collected from Guishan, Xiaolongtan and Yiliangdaogou coal mines in Yunnan Province. At first, the mixed bacteria in activated sludge were used to treat coal samples. The medium, culture temperature and pH were optimized in the process of treatment, but no obvious degradation effect of organic sulfur was observed. Eight strains of bacteria were isolated by infinite dilution method and selective medium using DBT as substrate. Morphological observation and 16s rRNA gene sequencing of 8 strains were carried out. According to the results of 16s rRNA gene sequence alignment, it was identified as Rhizobium sp., Xanthobacter sp., Rhodococcus erythropolisus, Rhodococcus sp., Pseudomonas sp. and Arthrobacter sp.). Moraxella sp. and Moraxella sp. The strain of Using DBT as substrate, the biodegradability of the 8 strains was evaluated by HPLC. It was found that 6 strains could degrade HWBHT2 benzothiopheneum. The DBT degradation rate of HWBHT2 bacteria was 45.8% after 3 days culture, and the DBT degradation rate of DBT was 76. 2% after 7 days culture. XFJ2 and HWBHT2 were used to treat Yunnan bituminous coal with organic sulfur removal. The results showed that the two strains could not degrade organic sulfur in Yunnan bituminous coal. Although the medium and physiological and biochemical conditions were improved and optimized, the content of organic sulfur in coal could not be reduced. The reason is that the organic sulfur in Yunnan bituminous coal may not be a DBT substance. Other components of Yunnan bituminous coal can inhibit the growth and metabolism of bacteria. In this study, six strains of bacteria capable of degrading DBT were isolated, and their ability to degrade DBT was studied, which provided a theoretical and practical basis for the further application of coal to remove organic sulfur. However, the removal of organic sulfur from coal by microorganism remains to be further studied.
【学位授予单位】：昆明理工大学
【学位级别】：硕士
【学位授予年份】：2015
【分类号】：TD849

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